High-efficiency Agrobacterium-mediated transformation of chrysanthemum via vacuum infiltration of internode  

作　　者：Sujuan Xu Ze Wu Jingya Zhao Fengjiao Zhang Xinqi Zhang Fadi Chen Nianjun Teng 

机构地区：[1]Key Laboratory of Landscaping Agriculture,Ministry of Agriculture and Rural Affairs/Key Laboratory of Biology of Ornamental Plants in East China,National Forestry and Grassland Administration,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China [2]Nanjing Agricultural University-Nanjing Oriole Island Modern Agricultural Development Co.,Ltd.Jiangsu Graduate Workstation,Nanjing 210043,China

出　　处：《Ornamental Plant Research》2022年第1期1-7,共7页观赏植物研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31672182,31171983);the Priority Academic Program Development of Jiangsu Higher Education Institutions;the Programs for New Century Excellent Talents in Universities,Ministry of Education of China(NCET-11-0669).

摘　　要：Leaf disc transformation is one of the traditional methods that are now widely used in chrysanthemum with highly economical and ornamental value in world flower production,but it depends on plant genotypes and is time consuming and complicated.In addition,the transformation success rate of this method is low,generally ranging from 0.1%to 6.25%.Therefore,a highly efficient transformation system is needed.In this study,we are the first to establish a high-efficient chrysanthemum Agrobacterium-mediated transformation system via vacuum infiltration.Chrysanthemum stem internode explants were used as research material and CmLEC1 was used as a reporter gene.After approximately 3 months of culture and selection,the positive transgenic plants were obtained.Additionally,the positive probability was about 42%.The transformation efficiency was up to 37.7%,and if the escapes were removed,it was 16%.Furthermore,stable expression of CmLEC1 in transgenic'Yuhualuoying'was confirmed by qRT-PCR analysis.These results suggest that this genetic transformation system via vacuum infiltration of chrysanthemum stem internode is highly efficient and convenient,and much better than traditional leaf disc transformation,and it will play an important role in chrysanthemum transformation and functional genetics research.

关 键 词：TRANSFORMATION VACUUM removed 

分 类 号：S682.11[农业科学—观赏园艺]