Transcriptome analysis of Clematis lanuginosa: Novel features of the molecular events occurring under heat-shock stress  

作　　者：Linfang Li Shuan Wang Qiao Li Linghe Sun Xin Han Lulu Gao Peng Wang Fenni Lv Rutong Yang Ya Li 

机构地区：[1]Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen),Nanjing 210014,China

出　　处：《Ornamental Plant Research》2022年第1期184-192,共9页观赏植物研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31800603);the Jiangsu Agricultural Science and Technology Innovation Fund[CX(20)3027];Jiangsu Key Laboratory for the Research and Utilization of Plant Resources(JSPKLB202203).

摘　　要：The weak heat tolerance of Clematis ornamental varieties negatively affects their ornamental qualities in the summer.To elucidate heat resistance mechanisms,Clematis lanuginosa,which is an important original parent of the Clematis large-flowered group of ornamental varieties,was selected for use in this study.Here,six libraries,including three biological replicates each of control and heat-shock stress samples,were determined using RNA-sequencing technology.In total,62,050 unigenes were obtained,and 6,439 unigenes exceeded 1 kb in length.A total of 42,377 unigenes were annotated using six databases.Between the two treatments,2,165 differentially expressed genes were identified,with 1,565 being up-regulated and 600 down-regulated.In addition,51 heat-shock protein-encoding genes were identified,among which the small heat-shock proteins accounted for 68.63%.In total,two heat-shock factors and 12 ribosomal proteins were significantly up-regulated under heatstress conditions.The differential expressions of ethylene-responsive transcription factor,chalcone synthase,cysteine-rich receptor-like kinase and aspartic protease unigenes in guard cells were induced by heat-shock.The data obtained will assist the elucidation of the molecular events underlying heat-stress responses in C.lanuginosa.

关 键 词：shock STRESS IPT 

分 类 号：Q94[生物学—植物学]