Identification of stress-related characteristics of the WRKY gene family: A case study of Dendrobium catenatum  

作　　者：Yining Zhao Yifei Zheng Luyuan Jiang Yunxia Niu Yanfang Yang Duanfen Chen Shance Niu 

机构地区：[1]College of Horticulture,Hebei Agricultural University,Baoding 071000,China [2]State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,The Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China [3]Department of Psychology,School of Vocational Education,Tianjin University of Technology and Education,Tianjin 300000,China [4]State Key Laboratory of North China Crop Improvement and Regulation,Hebei Agricultural University,Baoding 071001,China

出　　处：《Ornamental Plant Research》2022年第1期215-229,共15页观赏植物研究（英文）

基　　金：supported by the Young Talent Project of Hebei Agricultural University Foundation(YJ201848);the Youth Fund of Hebei Province Natural Science Foundation(C2019204295)。

摘　　要：As one of the largest families of transcription factors(TFs)in plants,the WRKY TF family plays a key role in regulating plant responses to various biotic and abiotic stresses.However,there is no confirmed method to quickly identify stress-responsive members from the WRKY gene family.In this study,all reported functional WRKY genes were first analyzed,and the amino acid patterns in response to stress were identified in group II-c(T-R/K-S/T-E/Q/D-V/I/L-E/D-I/V/H/N-L/M-D/E-D-G/E-F/Y-K/R-WRKYG-Q/K-K-A/T-VKN-S/N-P),group II-d(VPA-I/V-S-X-K-M/L/V/I-ADIP-P/A/V-D-D/EY/F-S-WRKYGQKPIKGSP-H/Y-PRGYYKCS-S/T-V/M-RGCPARKVER),and group II-e(PSD-S/A/L-WAWRKYGQKPIKGSPYPR-G/S-YYRCSSSKGC).WRKY genes in Dendrobium catenatum were used to validate the accuracy of these patterns.A total of 63 DcaWRKY genes were identified,their gene structures,conserved motifs,and gene expression patterns were analyzed,and a phylogenetic tree was constructed.Gene expression patterns were then analyzed under drought stress,and seven DcaWRKY genes(Dca002550,Dca002715,Dca005648,Dca007842,Dca010430,Dca016437,and Dca006787)were randomly selected to determine their expression levels and verify their expression patterns by quantitative real-time polymerase chain reaction analysis.The identified amino acid patterns were validated by drought-responsive WRKY genes in D.catenatum,confirming the accuracy of these amino acid patterns and providing valuable insights into further research of the WRKY family in D.catenatum.

关 键 词：stress DROUGHT FIR 

分 类 号：Q94[生物学—植物学]