Comparative transcriptome and proteome analysis of lily clones inoculated with Fusarium oxysporum f. sp. lilii  

作　　者：Yiping Zhang Xin Wang Chunlian Jin Feng Xu Xiumei Yang Yalian Jiang Lifang Zhang Lihua Wang Yan Su Lulin Ma Jihua Wang 

机构地区：[1]National Engineering Research Center for Ornamental Horticulture,Yunnan Flower Breeding Key Laboratory,Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650200,Yunnan,China [2]School of Agriculture,Yunnan University,Kunming 650200,Yunnan,China

出　　处：《Ornamental Plant Research》2022年第1期237-246,共10页观赏植物研究（英文）

基　　金：supported by National Key R&D Program of China(Grant No.2019YFD1000400,2020YFD10004);Kunming Comprehensive test station of national Flower Industry system(CARS-23-G56);National Natural Science Foundation of China(No.31260484);Yunnan Provincial Science and Technology Department(Grant No.2016BB009);the Green Food Brand-Build a Special Project(Floriculture)supported by Yunnan Provincial Finance Department(530000210000000013742).

摘　　要：Basal bulb rot is the major factor restricting the production of lily,caused by Fusarium oxysporum f.sp.lilii.To systematically analyze the transcriptomic and proteomic responses of lily clones to F.oxysporum,we constructed six RNA-seq libraries and four iTRAQ proteomic libraries using lily resistant and susceptible clones sampled at 0,24 and 48 h post-inoculation(hpi)with F.oxysporum respectively.137,715 unigenes were generated,of which 7,667 were differentially expressed.1,679 and 4,051 differentially expressed genes(DEGs)between resistant and susceptible clones were isolated from samples collected at 0 and 48 hpi.Four hundred and thirty three and 155 DEGs were identified in resistant clones sampled at 24 and 48 hpi separately while 550 and 799 DEGs were isolated in the susceptible clones sampled at 24 and 48 hpi respectively.The results of iTRAQ analysis revealed 7,482 proteins in resistant and susceptible clones.Data analysis of transcriptome and proteome indicated that 5,735 proteins corresponded to mRNAs.Three hundred and sixteen and 1,052 DEGs had corresponding DEPs.At 48 hpi,the resistant clones showed 155 DEGs and 108 corresponding DEPs,while the susceptible clones showed 799 DEGs and 316 corresponding DEPs.In general,these results enhance comprehension of the defense response of lily resistant clones to F.oxysporum infection and provide valuable sequence data for studying the resistance mechanism.

关 键 词：OXYSPORUM analysis LILY 

分 类 号：S43[农业科学—农业昆虫与害虫防治]