LY294002联合顺铂通过细胞自噬效应抑制人喉癌HEP-2细胞的生长  

LY294002 combined with cisplatin inhibits the growth of human laryngeal carcinoma HEP-2 cells by autophagy

作　　者：姜玉秋[1] 王頔 姚远[2] 闫智永[1] 张爽[1] JIANG Yuqiu;WANG Di;YAO Yuan;YAN Zhiyong;ZHANG Shuang(Department of Otolaryngology,The Second Affiliated Hospital of Shenyang Medical College,Shenyang 110002,China;Department of Oncology,The People′s Hospital of Liaoning Province)

机构地区：[1]沈阳医学院附属第二医院耳鼻喉科,辽宁沈阳110002 [2]辽宁省人民医院肿瘤二科

出　　处：《沈阳医学院学报》2023年第3期243-247,共5页Journal of Shenyang Medical College

基　　金：沈阳医学院科技基金项目(No.20177055)。

摘　　要：目的:探讨PI3K/AKT通路抑制剂LY294002联合顺铂抑制人喉癌HEP-2细胞生长的机制。方法:采用MTT及细胞活力检测单独LY294002处理对HEP-2细胞的毒性作用,并且观察LY294002联合顺铂对HEP-2细胞产生的影响;采用免疫荧光检测LY294002联合顺铂对HEP-2细胞内自噬特异性标志蛋白LC3B表达的诱导作用;采用实时荧光定量PCR(RT-qPCR)检测LY294002联合顺铂处理HEP-2细胞后相关自噬因子的表达。结果:单独LY294002对喉癌HEP-2细胞无明显毒性作用,而1μg/ml顺铂联合2μg/ml LY294002可显著增强顺铂杀伤HEP-2细胞的效能,并且明显刺激LC3B的表达。RT-qPCR结果显示,1μg/ml顺铂联合2μg/ml LY294002作用显著升高细胞中自噬标志物ATG3、ATG4A、ATG4B、ATG4C、ATG5和ATG7 mRNA的表达。结论:顺铂联合LY294002可能通过激活细胞自噬抑制喉癌HEP-2细胞生长的作用。Objective:To investigate the mechanism of the inhibitory effect of LY294002 combined with cisplatin on the growth of human laryngeal carcinoma HEP-2 cells.Methods:MTT and cell viability were used to detect the cytotoxic effect of LY294002 alone and LY294002 combined with cisplatin on HEP-2 cells.The effect of LY294002 combined with cisplatin on autophagic marker LC3B in HEP-2 cells was detected by immunofluorescence.And the expressions of autophagic factors in autophagy pathway were detected by real-time quantitative PCR(RT-qPCR).Results:LY294002 alone had no significant cytotoxic effect on HEP-2 cells,while 2μg/ml LY294002 combined with 1μg/ml cisplatin significantly enhanced the killing effect of cisplatin on the cells,and greatly increased the expression of LC3B.The results of RT-qPCR showed that LY294002 combined with cisplatin significantly increased the mRNA expression of ATG3,ATG4A,ATG4B,ATG4C,ATG5 and ATG7.Conclusion:The combination of LY294002 and cisplatin may inhibit the growth of HEP-2 cells by activating autophagy.

关 键 词：LY294002 顺铂 喉癌 细胞自噬 磷脂酰肌醇-3激酶 LC3B 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R977.12[医药卫生—基础医学]