一株产紫杉醇真菌的分离与鉴定  

Isolation and identification of a paclitaxel-producing fungus

作　　者：鹿孟云 陈金凤 马玉涵 祝嫦巍 LU Mengyun;CHEN Jinfeng;MA Yuhan;ZHU Changwei(College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China;College of Life and Health Science,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院农学院,安徽凤阳233100 [2]安徽科技学院生命与健康科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2023年第2期46-50,共5页Journal of Anhui Science and Technology University

基　　金：安徽省高校自然科学研究项目(KJ2020A0060,2022AH051624);安徽科技学院功能农业专项(2021gnny06);安徽科技学院科研人才稳定项目(SKWD201902)。

摘　　要：目的:从自然界分离筛选具有紫杉醇合成能力的菌株,并对该菌株进行初步鉴定。方法:用常规的微生物分离方法分离真菌,通过薄层色谱法和高效液相色谱法检测发酵液中是否含有紫杉醇,通过ITS序列分析鉴定菌种。结果:分离获得一株具有紫杉醇合成能力的真菌(T20),ITS序列分析结果显示该菌与拟盘多毛孢属(Pestalotiopsis)真菌高度同源,但聚类分析结果显示,该菌不能与目前已知的菌株聚为一类。结论:分离获得一株新的具有紫杉醇合成能力的真菌,为产紫杉醇真菌的资源开发提供数据参考。Objective:Isolation and identification fungi strains with paclitaxel synthesis ability from nature were investigated.Methods:The fungi were isolated by conventional microbial isolation methods,and fermentation samples were detected by thin-layer chromatography and high performance liquid chromatography for their ability to synthesize paclitaxel.The strains were identified by ITS sequence analysis.Results:A fungus strain with paclitaxel synthesis ability was isolated and named T20.ITS sequence analysis showed that the fungus was highly similar to the species in genus Pestalotiopsis.However,the phylogenetic analysis showed that the fungus could not be clustered with the currently known strains.Conclusion:Isolation of a new strain with paclitaxel synthesis ability,which may provide data references for the resource development of paclitaxel-producing fungi.

关 键 词：真菌 紫杉醇 拟盘多毛孢属 

分 类 号：Q93[生物学—微生物学]