纳米胶束中阿霉素和槲皮素含量测定方法的建立  

Establishment of a methodology for the determination of TPP-DOX and Que in nano micelles in vitro

作　　者：朱姣姣 郭秀春[1] 陈云龙 邓勇 彭先飞 周丕俊 蒲晓辉[1] ZHU Jiaojiao;GUO Xiuchun;CHEN Yunlong;DENG Yong;PENG Xianfei;ZHOU Pijun;PU Xiaohui(Sehool of Pharmacy,Henan University,Kaifeng 475004,CHN)

机构地区：[1]河南大学药学院,河南开封475004

出　　处：《河南大学学报（医学版）》2023年第2期96-101,共6页Journal of Henan University:Medical Science

基　　金：国家自然科学基金(U190410650);河南大学学生创新创业训练计划项目(20211020006)。

摘　　要：目的:建立纳米胶束中三苯基膦-阿霉素和槲皮素含量的高效液相色谱测定方法。方法:纳米胶束中三苯基膦-阿霉素和槲皮素含量的测定采用Hypersil C18色谱柱(250×4.6 mm,5μm),以纯甲醇为流动相A,以0.3%磷酸水溶液(pH 3.0)为流动相B,梯度洗脱(0~9 min,60%A,检测波长λ=373 nm;9~10 min,60%A→80%A;10~15 min,80%A,检测波长λ=480 nm);流速:1.0 mL·min^(-1);柱温:30℃;进样量:20μL。结果:]三苯基膦-阿霉素的线性回归方程为Y=4153.3x+8505.2(R^(2)=0.9999),线性范围为2~64μg·mL^(-1),槲皮素线性回归方程为Y=81230x-23677(R^(2)=0.9999),线性范围为0.5~40μg·mL^(-1)。三苯基膦-阿霉素和槲皮素在线性范围内线性关系良好。在保留时间内未见其他干扰峰,该方法的精密度、稳定性、方法回收率和加样回收率均较好。结论:该方法可用于同时测定三苯基膦-阿霉素和槲皮素的含量。Objective:To establish a method for the determination of TPP-DOX and Que in nanomicelles by HPLC.Methods:Hypersil C18 column(250×4.6 mm,5μm)was used.0-9min mobile phase was methanol:0.3%phosphoric acid(pH 3.0)60;40(V/V)detection wavelength λ=373 nm,10-15 min mobile phase was methanol:0.3%phosphoric acid(pH 3.0)80:20(V/V)detection wavelength λ=480 nm flow rate:1.0mL/min;Column temperature:30 ℃;Flow rate:1.0 mL./min;Iniection volume:20 pL.The precision,stability,method recovery and sample recovery of the method were investigated,and the drug loading and encapsulation rate of the nanomicelles were also determined.The linear regression equation of TPP-DOX was Y=4153.3x+8505.2(R^(2)=0.9999),and the linear range was2~64 ug·mL^(-1).Que linear regression equation is Y-81230X-23677(R^(2)=0.9999),the linear range is 0.5~40 ug·mL^(-1).Results:There was a good linear relationship between the peak arca and concentration of TPP-D0X and Que in linear range.No other interference peak was observed during the retention time.The method has good precision,stability,method recovery and addition recovery,Conclusion:This method can be used for simultaneous determination of TPP-DOX and Que.

关 键 词：三苯基磷-阿霉素 槲皮素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]