猪白细胞介素4的基因克隆及其在囊尾蚴DNA疫苗中的应用  

Clone of porcine IL-4 cDNA and its application in DNA vaccine of Cysticercus cellulosae

作　　者：吴丹[1] 郭瀛军[1] 王庆敏[1] 孙树汉[1] 

机构地区：[1]第二军医大学基础医学部医学遗传学教研室,上海200433

出　　处：《第二军医大学学报》2002年第11期1192-1194,共3页Academic Journal of Second Military Medical University

基　　金：国家863计划资助项目(2001AA213111).

摘　　要：目的:克隆猪白细胞介素4(IL-4)cDNA,观察其在抗囊尾蚴免疫中的疫苗佐剂作用。方法:通过RT-PCR法获得带有5'AUG侧翼翻译优化突变序列的猪IL-4 cDNA,测序后重组入真核表达载体pcDNA,在体外瞬时表达的基础上与囊尾蚴保护性抗原DNA疫苗联合免疫仔猪。结果:获得的猪IL-4 cDNA序列与文献报道的完全一致,在体外瞬时表达中获得了有生物学活性的猪IL-4。其与囊尾蚴保护性抗原DNA疫苗联合应用可有效提高个体体液免疫应答水平。结论:构建了一高效表达猪IL-4的真核表达质粒,初步实验证实了其在抗囊尾蚴DNA疫苗中的佐剂效应。Objective: To clone porcine IL-4 cDNA and observe its adjuvanticity of vaccine against T. solium cysticerco-sis. Methods:The cDNA of porcine IL-4 was amplified by RT-PCR, which had 5' AUG initiatory codon with optimized trans-lational initiation. After sequencing,the cDNA was intergrated into expression vector pcDNA and transient expression was performed. Then newborn pigs were immunized with IL-4 expression vector and protective antigen DNA vaccine against T. solium cysticercosis. Results:The obtained sequence of porcine IL-4 cDNA was the same as reported. IL-4 and protective antigen could effectively promote the humoral response. Conclusion:An efficient expression plasmid containing porcine IL-4 cDNA is constructed. Its adjuvanticity of DNA vaccine against T. solium cysticercosis is preliminarily conformed.

关 键 词：猪白细胞介素4 基因克隆 囊尾蚴 DNA疫苗 佐剂作用 

分 类 号：R392－33[医药卫生—免疫学]