Are EPB41 and alpha-synuclein diagnostic biomarkers of sport-related concussion?Findings from the NCAA and Department of Defense CARE Consortium  

作　　者：Rany Vorn Christina Devoto Timothy B.Meier Chen Lai Sijung Yun Steven P.Broglio Sara Mithani Thomas W.McAllister Christopher C.Giza Hyung-Suk Kim Daniel Huber Jaroslaw Harezlak Kenneth L.Cameron Gerald McGinty Jonathan Jackson Kevin M.Guskiewicz Jason P.Mihalik Alison Brooks Stefan Duma Steven Rowson Lindsay D.Nelson Paul Pasquina Michael A.McCrea Jessica M.Gill the CARE Consortium Investigators 

机构地区：[1]Johns Hopkins School of Nursing and Medicine,Baltimore,MD 21205,USA [2]National Institutes of Health,Bethesda,MD 20892,USA [3]Department of Neurosurgery,Medical College of Wisconsin,Milwaukee,WI 53226,USA [4]Predictiv Care,Inc.,Mountain View,CA 94086,USA [5]Michigan Concussion Center,University of Michigan,Ann Arbor,MI 48109,USA [6]Department of Psychiatry,Indiana University School of Medicine,Indianapolis,IN 46202,USA [7]Departments of Pediatrics and Neurosurgery,University of California at Los Angeles(UCLA),Los Angeles,CA 90024,USA [8]Department of Epidemiology and Biostatistics School of Public Health–Bloomington,Indiana University,Bloomington,IN 47405,USA [9]John A.Feagin Sports Medicine Fellowship,Keller Army Community Hospital,West Point,NY 10996,USA [10]United States Air Force Academy,Colorado Springs,CO 80840,USA [11]Mathew Gfeller Center,Department of Exercise and Sport Science,University of North Carolina at Chapel Hill,Chapel Hill,NC 27514,USA [12]Department of Orthopedics,Division of Sports Medicine,School of Medicine and Public Health,University of Wisconsin,Madison,WI 53705,USA [13]Department of Biomedical Engineering and Mechanics,Virginia Tech,Blacksburg,VA 24061,USA [14]Center for Neuroscience&Regenerative Medicine,Uniformed Services University,Bethesda,MD 20814,USA [15]不详 [16]Current affiliation:School of Nursing,University of Texas Health Science Center San Antonio,San Antonio,TX 78229,USA

出　　处：《Journal of Sport and Health Science》2023年第3期379-387,共9页运动与健康科学（英文）

基　　金：supported by the Grand Alliance CARE Consortium;funded in part by the National Collegiate Athletic Association(NCAA);the Department of Defense(DoD).;supported by the Office of the Assistant Secretary of Defense for Health Affairs,through the Combat Casualty Care Research Program,endorsed by the Department of Defense,under Award No.W81XWH1420151。

摘　　要：Background:Current protein biomarkers are only moderately predictive at identifying individuals with mild traumatic brain injury or concussion.Therefore,more accurate diagnostic markers are needed for sport-related concussion.Methods:This was a multicenter,prospective,case-control study of athletes who provided blood samples and were diagnosed with a concussion or were a matched non-concussed control within the National Collegiate Athletic Association-Department of Defense Concussion Assessment,Research,and Education Consortium conducted between 2015 and 2019.The blood was collected within 48 h of injury to identify protein abnormalities at the acute and subacute timepoints.Athletes with concussion were divided into 6 h post-injury(0-6 h post-injury)and after 6 h postinjury(7-48 h post-injury)groups.We applied a highly multiplexed proteomic technique that used a DNA aptamers assay to target 1305proteins in plasma samples from athletes with and without sport-related concussion.Results:A total of 140 athletes with concussion(79.3%males;aged 18.71±1.10 years,mean±SD)and 21 non-concussed athletes(76.2%males;19.14±1.10 years)were included in this study.We identified 338 plasma proteins that significantly differed in abundance(319 upregulated and 19 downregulated)in concussed athletes compared to non-concussed athletes.The top 20 most differentially abundant proteins discriminated concussed athletes from non-concussed athletes with an area under the curve(AUC)of 0.954(95%confidence interval:0.922-0.986).Specifically,after 6 h of injury,the individual AUC of plasma erythrocyte membrane protein band 4.1(EPB41)and alpha-synuclein(SNCA)were 0.956 and 0.875,respectively.The combination of EPB41 and SNCA provided the best AUC(1.000),which suggests this combination of candidate plasma biomarkers is the best for diagnosing concussion in athletes after 6 h of injury.Conclusion:Our data suggest that proteomic profiling may provide novel diagnostic protein markers and that a combination of EPB41 and SNCA is the most predictive

关 键 词：Biomarkers College athletes CONCUSSION Mild traumatic brain injury Sport injury 

分 类 号：G804.2[文化科学—运动人体科学] R651.15[文化科学—体育学]