外源油菜素内酯引发对劣变燕麦种子抗氧化特性的影响  

Characteristics of Deteriorated Oat Seeds

作　　者：王勃 夏方山 董秋丽 岑慧芳 李尹琳 张杰敏 WANG Bo;XIA Fang-shan;DONG Qiu-li;CEN Hui-fang;LI Yin-lin;ZHANG Jie-min(College of Grassland Science,Shanxi Agricultural University,Taigu,Shanxi Province 030801,China)

机构地区：[1]山西农业大学草业学院,山西太谷030801

出　　处：《草地学报》2023年第5期1461-1468,共8页Acta Agrestia Sinica

基　　金：山西农业大学“十四五”生物育种工程项目(YZGC134);山西省重点研发计划项目(202102140601006,201903D221091);内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303)资助。

摘　　要：为探讨外源油菜素内酯引发对劣变种子抗氧化能力的影响,试验采用燕麦(Avena sativa)种子为材料,人工老化后经不同浓度(0,0.01,0.10,0.50,1.00,10.00μmol·L^(-1))的油菜素内酯(Brassinosteroids,BR)溶液引发0(CK),1,6,12,18和24 h,分析了其发芽率、超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)、谷胱甘肽还原酶(glutathione reductase,GR)活性及丙二醛(Maloddialdehyde,MDA)含量的变化规律。结果表明:低浓度(0.01~0.50μmol·L^(-1))的BR可提高燕麦种子的发芽率,增加其SOD,CAT,APX和GR活性,降低其MDA含量;而BR浓度为10.00μmol·L^(-1)时(除引发18 h外),其发芽率、CAT、APX和GR活性均低于CK,增加MDA含量。浓度为0.01μmol·L^(-1)的油菜素内酯引发24 h可能是缓解燕麦种子老化的最佳处理。In order to explore the effect of exogenous brassinosteroid priming on the antioxidant capacity of deteriorated seeds,Oat(Avena sativa)seeds were used as materials,and tested them with different concentrations(0,0.01,0.10,0.50,1.00,10.00μmol·L^(-1))brassinosteroid solution initiated for 0(CK),1,6,12,18 and 24 h durations.The changes of germination rate,superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),glutathione reductase(GR)and malondialdehyde(MDA)content were analyzed.The results showed that low concentration(0.01~0.50μmol·L^(-1))of BR improved the germination rate of oat seeds,and increase the activities of SOD,CAT,APX and GR,and reduce the content of MDA in the oat seeds.When the concentration of BR reached 10.00μmol·L^(-1)(except for 18 h initiation),the germination rate,CAT,APX and GR activities were lower than those of the seeds in CK,and the MDA content was higher.Priming with 0.01μmol·L^(-1)brassinolide for 24 h may be the best treatment to alleviate the aging of oat seeds.

关 键 词：油菜素内酯 燕麦 种子老化 种子引发 抗氧化性能 

分 类 号：S152.6[农业科学—土壤学]