冠通方对急性心肌梗死大鼠心肌血管新生的影响  

Effect of Guantong Decoction on Myocardial Angiogenesis in Rats after Acute Myocardial Infarction

作　　者：杨成龙[1,2] 方敏 刘建和 梁昊[4,5] Yang Chenglong;Fang Min;Liu Jianhe;Liang Hao(The First Affiliated Hospital of Hunan University of Chinese Medicine,Hunan,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]国家中医心血管病临床医学研究中心分中心,湖南长沙410007 [3]宁夏医科大学,宁夏银川750000 [4]湖南中医药大学,湖南长沙410007 [5]中医诊断研究所,湖南长沙410007

出　　处：《中国中医急症》2023年第5期791-795,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖南省中医药管理局中医药科研基金青年项目(2021239)。

摘　　要：目的 观察冠通方对急性心肌梗死(AMI)大鼠缺血心肌的保护作用及对血管新生的影响。方法 将SD大鼠随机分为空白对照组、模型组、麝香通心滴丸组和冠通方组,除对照组外,其余各组大鼠均采用左冠状动脉前降支结扎法建立AMI模型,对照组只穿线不结扎。造模成功后,麝香通心滴丸组每日予麝香通心滴丸灌胃,冠通方组每日予冠通方灌胃,其余各组大鼠予等体积生理盐水灌胃,连续14 d。称大鼠体质量及心脏质量,计算心脏/体质量比;HE染色法评价大鼠心肌组织损伤程度;免疫荧光法测定大鼠心梗边缘区CD34阳性表达及计数微血管数目(MVC);免疫组化测定大鼠心肌组织血管内皮生长因子(VEGF)蛋白表达。结果 与空白对照组比较,模型组心脏/体质量比明显增加(P <0.01);模型组HE染色显示心肌细胞结构紊乱,细胞碎裂、坏死,间质充血和炎性细胞浸润现象;免疫荧光检测示心肌组织CD34表达及MVC升高(P <0.01);免疫组化检测示心肌组织VEGF蛋白表达增加(P <0.01)。与模型组比较,冠通方组心脏/体质量比明显下降(P <0.01);CD34表达及MVC显著升高(P <0.01);VEGF蛋白表达显著增加(P <0.01)。结论 冠通方可通过促进缺血区血管新生来减轻心肌细胞损伤,保护受损心肌。Objective:To discuss the protective effect of Guantong Decoction on myocardium and its effect on angiogenesis in rats with acute myocardial infarction(AMI).Methods:Rats were randomly divided into the control group,model group,Shexiang Tongxin dripping pills group and Guantong Decoction group.In addition to the control group,AMI model was established by ligation of left anterior descending coronary artery.The control group only received threading without ligation.After the model was successfully established,the rats in the Shexiang Tongxin dripping pills group were given gavage of Shexiang Tongxin dripping pills daily,and the rats in the Guantong Decoction group were given gavage of Guantong Decoction daily,and the rats in the other groups were given gavage of equal volume of normal saline.Continuous gavage was performed for 14 days.The body weight and heart weight of rats were weighed,and the heart/body weight ratio was calculated.HE staining was used to evaluate the degree of myocardial injury.Detection of CD34 positive expression and micro-vessel counting(MVC)in the marginal zone of myocardial infarction in rats were conducted by immunofluorescence.The expression of VEGF protein in myocardial tissue was detected by immunohistochemistry.Results:Compared with the control group,the hear/body weight ratio of the model group was significantly increased(P<0.01);HE staining showed myocardial cell structure disorder,cell fragmentation,necrosis,interstitial congestion and inflammatory cell infiltration;immunofluorescence showed that the CD34 and MVC increased in myocardial tissue(P<0.01);immunohistochemistry showed that the expression of VEGF protein in myocardial tissue increased(P<0.01).Compared with the model group,the heart/body weight ratio of the Guantong Decoction group was significantly decreased(P<0.01);CD34 and MVC were significantly increased(P<0.01);VEGF protein was significantly increased(P<0.01).Conclusion:Guantong Decoction can reduce myocardial cell injury and protect damaged myocardium by promoting angi

关 键 词：急性心肌梗死 冠通方 血管新生 血管内皮生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]