新生隐球菌高效转化方法的研究  

Highly effective transformation of Cryptococcus neoformans

作　　者：潘炜华[1] 廖万清[1] 顾菊林[1] 霍克克[2] 

机构地区：[1]第二军医大学长征医院皮肤性病科,上海200003 [2]复旦大学遗传学研究所酵母组7

出　　处：《第二军医大学学报》2002年第11期1218-1220,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目(39570043).

摘　　要：目的:探索并建立高效的新生隐球菌转化方法.方法:采用化学转化法进行新生隐球菌的转化,利用特定试剂使隐球菌在一定环境中能摄取外源DNA,并与电转化法进行比较;利用酵母质粒稳定性测定鉴定该化学转化方法的实用性.结果:用化学转化的酵母每μg质粒DNA转化效率>1 000个转化子,其转化效率远高于传统所用的电转化方法(每μg质粒DNA 20～30个转化子).结论:在新生隐球菌的转化系统中寻找到一个合适的化学转化条件,为后续研究奠定了实验基础.Objective: To establish a highly effective transformation method of Cryptococcus neoformans. Methods: Special reagents was used to make C. neoformans take in external DNA under given condition. This chemical transformation result was compared with that of electrotransformation. The feasibility of this chemical transformation was tested by plasmid stability test. Results: The efficiency of this chemical transformation was more than 103 transformants/μg plasmid DNA, far more than that of the traditional electrotransformation. Conclusion: An appropriate transformation method is established for C. neoformans transformation, which has high transformation efficiency.

关 键 词：新生隐球菌 转化 化学转化法 电转化法 

分 类 号：R379.5[医药卫生—病原生物学]