Tyrosine promotes anthocyanin biosynthesis in pansy(Viola×wittrockiana)by inducing ABA synthesis  

作　　者：Zheng Cui Jia Gu Jie Li Anjin Zhao Yingge Fu Tongxin Wang Tingge Li Xueqing Li Yuhui Sheng Ying Zhao Xiqiang Song Yang Zhou Ting Peng Jian Wang 

机构地区：[1]Key Laboratory of Genetics and Germplasm Innovation of Tropical Forest Trees and Ornamental Plants,Ministry of Education,Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan,College of Forestry,Hainan University,Haikou,Hainan 570228,China [2]Guizhou University,Guiyang,Guizhou 550025,China [3]Hengxing University,Qingdao,Shandong 266100,China

出　　处：《Tropical Plants》2022年第1期73-84,共12页热带植物（英文刊）

基　　金：supported by the National Natural Science Foundation of China(Grant No.32160719,32060365 and 31760590);the Natural Science Foundation of Guizhou Province(No.ZK[2022]095);the Cultivation Research Program of Guizhou University(No.[2018]5781).

摘　　要：Viola×wittrockiana(pansy)is an important ornamental plant,particularly during winter and spring.In previous studies,we found that the tyrosine decarboxylase gene of pansy(VwTYDC)was expressed differently in blotched and non-blotched areas of pansy petals,suggesting that tyrosine may have a role in anthocyanin biosynthesis.In this study,we found that virus-induced gene silencing of VwTYDC caused an accumulation of pink pigmentation in pansy petals.Likewise,exogenous tyrosine treatment(TYRT)induced the formation of black stripes in nonblotched petal areas.Metabolome analysis indicated that the contents of two anthocyanins,cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside,increased significantly in the TYRT areas.RT-qPCR results revealed that the anthocyanin-related genes VwHCT,VwC3′H,VwCHS,and VwUGT were upregulated in the same areas.Transcriptome analysis revealed that four genes involved in the abscisic acid(ABA)biosynthesis pathway(VwNCED,VwABA2,VwAAO3,and VwCYP707A)were significantly upregulated in the same TYRT areas.ABA content was measured by ESI-HPLCMS/MS,and ABA content was significantly higher in TYRT areas than in control areas.In addition,when exogenous ABA was spread onto nonblotched petal areas,anthocyanin biosynthesis genes were upregulated just as with tyrosine.Thus,transcriptome and metabolite analyses revealed a possible novel regulatory network for anthocyanin biosynthesis in which tyrosine induces ABA synthesis and ABA then promotes anthocyanin biosynthesis in pansy petals.

关 键 词：HPLCMS/MS SYNTHESIS addition 

分 类 号：Q94[生物学—植物学]