生物基塑料单体5-氨基戊酸的生物合成新途径  

A new biosynthesis route for production of 5-aminovalanoic acid,a biobased plastic monomer

作　　者：康雅琦 罗若诗 林凡祯 程杰 周桢 王丹[1,2] KANG Yaqi;LUO Ruoshi;LIN Fanzhen;CHENG Jie;ZHOU Zhen;WANG Dan(School of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400044,China;State Key Laboratory of Coal Mine Disaster Dynamics and Control,Chongqing 400044,China;School of Pharmacy,Chongqing University,Chongqing 400044,China)

机构地区：[1]重庆大学化学化工学院,重庆400044 [2]煤矿灾害动力学与控制国家重点实验室,重庆400044 [3]重庆大学药学院,重庆400044

出　　处：《生物工程学报》2023年第5期2070-2080,共11页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2022YFC2105700,2021YFC2103300,2021YFE0190800);国家自然科学基金(21978027);中央高校基本科研业务费专项资金(2022CDJXY-003,2022CDJHLW006,2020CDCGJ020,2020CDCGHG068);重庆市杰出青年基金(cstc2021jcyj-jqX0013);重庆市教育委员会科学技术研究计划青年项目(KJQN201900112)。

摘　　要：5-氨基戊酸(5-aminovalanoic acid,5AVA)可作为新型塑料尼龙5和尼龙56的前体,是合成聚酰亚胺的有前途的平台化合物。目前5-氨基戊酸的生物合成法普遍产率较低且合成过程复杂,成本高。为实现5AVA的绿色生物合成,本研究通过组合表达来自日本白腹鲭(Scomber japonicas)的L-赖氨酸α-氧化酶、来自乳酸乳球菌(Lactococcus lactis)的α-酮酸脱羧酶和来自大肠杆菌(Escherichia coli)的醛脱氢酶,在大肠杆菌中建立了一条以L-赖氨酸为原料,以2-酮-6-氨基己酸盐为中间产物生物合成5AVA的途径。在葡萄糖浓度为55 g/L,赖氨酸盐酸盐40 g/L的初始条件下,最终消耗158 g/L的葡萄糖和144 g/L的赖氨酸盐酸盐,补料分批发酵产生了57.52 g/L的5AVA,摩尔得率为0.62 mol/mol。与文献报道的以2-酮-6-氨基己酸盐为中间产物的5AVA生物合成途径相比,本文报道的新途径无需使用乙醇和双氧水,且5AVA产量进一步提高。5-aminovalanoic acid(5AVA)can be used as the precursor of new plastics nylon 5 and nylon 56,and is a promising platform compound for the synthesis of polyimides.At present,the biosynthesis of 5-aminovalanoic acid generally is of low yield,complex synthesis process and high cost,which hampers large-scale industrial production.In order to achieve efficient biosynthesis of 5AVA,we developed a new pathway mediated by 2-keto-6-aminohexanoate.By combinatory expression of L-lysineα-oxidase from Scomber japonicus,α-ketoacid decarcarboxylase from Lactococcus lactis and aldehyde dehydrogenase from Escherichia coli,the synthesis of 5AVA from L-lysine in Escherichia coli was achieved.Under the initial conditions of glucose concentration of 55 g/L and lysine hydrochloride of 40 g/L,the final consumption of 158 g/L glucose and 144 g/L lysine hydrochloride,feeding batch fermentation to produce 57.52 g/L of 5AVA,and the molar yield is 0.62 mol/mol.The new 5AVA biosynthetic pathway does not require ethanol and H2O2,and achieved a higher production efficiency as compared to the previously reported Bio-Chem hybrid pathway mediated by 2-keto-6-aminohexanoate.

关 键 词：5-氨基戊酸 L-赖氨酸盐酸盐 人工途径 代谢工程 

分 类 号：TQ226.36[化学工程—有机化工] TQ320.4