小粒材小蠹伴生真菌的分离鉴定及其致病性研究  

Isolation,Identification and Pathogenicity of Fungi Associated with Xyleborus saxeseni

作　　者：梁甜 章宇 巨云为 廖菘凯 周洁璐 LIANG Tian;ZHANG Yu;JU Yun-wei;LIAO Song-kai;ZHOU Jie-lu(College of Forestry,Nanjing Forestry University,Co-innovation Center for the Sustainable Forestry in Southern China,Nanjing 210037,Jiangsu,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,Guangdong,China)

机构地区：[1]南京林业大学林学院,南方现代林业协同创新中心,江苏南京210037 [2]华南农业大学林学与风景园林学院,广东广州510642

出　　处：《西北林学院学报》2023年第3期123-129,共7页Journal of Northwest Forestry University

基　　金：江苏省林业科技创新与推广项目(LYKJ[2021]27,LYKJ[2021]1)。

摘　　要：为明确树状月季害虫小粒材小蠹伴生真菌的种类及其致病性,采用组织分离法分别对小粒材小蠹的成虫、幼虫、坑道、寄主变色边材和维管束进行真菌分离培养,结合形态学和分子生物学对所得菌株进行分类鉴定,通过柯赫氏法则进一步验证伴生菌的致病性。结果表明,5种分离材料共分离出伴生真菌10株,其中腐皮镰刀菌在不同分离材料中的分离率均为最高,是小粒材小蠹伴生菌的优势种群;腐皮镰刀菌、层出镰刀菌、藤仓镰刀菌对月季均有致病性,接种后的月季叶片和茎干出现病斑,叶片发病率分别为89.73%、34.15%、63.24%,发病面积分别为1.62、0.20、0.28 cm2;茎干发病率分别为55.56%、27.78%、30.00%,变色反应区长度分别为4.74、2.98、4.33 cm。该结果为进一步揭示伴生真菌与小粒材小蠹的相互作用关系提供了基础,为小粒材小蠹的综合防治提供科学的理论依据。The aim of this study was to identify species and pathogenicity of fungi associated with Xyleborus saxeseni,a pest of tree rose.The fungi were isolated and cultured from adults,larvae,tunnels,discolored sapwood and vascular bundles.The obtained strains were classified and identified based on morphological and molecular biological features.The pathogenicity was tested by Koch's rule.Ten 10 fungi were isolated in total,and Fusarium solani had the highest incidence and was dominant among all the isolated materials.The inoculation to rose plants revealed that F.solani,F.proliferatum and F.fujikuroi were all pathogenic.Disease spots appeared in the leaves and stems of rose after inoculation,and leaf incidences were 89.73%,34.15%and 63.24%,respectively,and disease areas were 1.62,0.20,and 0.28 cm 2,respectively.Incidences of stem were 55.56%,27.78%and 30.0%,respectively,and lesion length of discoloration were 4.74,2.98,and 4.33 cm,respectively.The results further reveal the interaction between associated fungi and X.saxeseni,and provide a theoretical basis for integrated control of this beetle.In addition,it is also the first time to report X.saxeseni as a pest on rose.

关 键 词：树状月季 小粒材小蠹 伴生真菌 分离鉴定 致病性 

分 类 号：S763.15[农业科学—森林保护学]