Cerium oxide nanozymes alleviate oxidative stress in tenocytes for Achilles tendinopathy healing  

作　　者：Xingquan Xu Rongliang Wang Yixuan Li Rui Wu Wenjin Yan Sheng Zhao Quanyi Liu Yan Du Wenli Gong Weitong Li Hui Wei Dongquan Shi 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology,Division of Sports Medicine and Adult Reconstructive Surgery,Department of Orthopedic Surgery,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing 210008,China [2]Department of Biomedical Engineering,College of Engineering and Applied Sciences,Nanjing National Laboratory of Microstructures,Jiangsu Key Laboratory of Artificial Functional Materials,Nanjing University,Nanjing 210023,China [3]State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China [4]University of Science and Technology of China,Hefei 230026,China [5]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Chemistry and Biomedicine Innovation Center(ChemBIC),Nanjing University,Nanjing 210023,China

出　　处：《Nano Research》2023年第5期7364-7372,共9页纳米研究（英文版）

基　　金：the National Natural Science Foundation of China(Nos.81941009,81991514,32271409,and 82202778);Nanjing Distinguished Youth Fund(No.JQX20001);Jiangsu Provincial Key R&D Program(No.BE2022836);China Postdoctoral Science Foundation(No.2020M671456);National Basic Research Program of China(No.2021YFA1201404);Jiangsu Provincial Key Medical Center Foundation,Jiangsu Provincial Medical Outstanding Talent Foundation,Jiangsu Provincial Medical Youth Talent Foundation and Jiangsu Provincial Key Medical Talent Foundation,and the Fundamental Research Funds for the Central Universities(Nos.14380493 and 14380494).

摘　　要：Background:Reactive oxygen species(ROS)is considered as ubiquitous and highly active chemicals that influence tendon integrity and orchestrate tendon repair.With significant recent advances in nanomaterials,cerium oxide nanoparticles(CeO_(2)NPs)exhibit superoxide dismutase-and catalase-like activities.Herein,we introduced a therapeutic approach of CeO_(2)NPs for Achilles tendinopathy(AT)healing.Methods:CeO_(2)NPs were synthesized to examine their effect as ROS scavengers on AT healing in vitro and in vivo.The mRNA levels of inflammatory factors were evaluated in AT after CeO_(2)NPs treatment in vitro.The mechanisms underlying CeO_(2)NPs-mediated stimulation of NRF2 translocation and ERK signaling were verified through immunofluorescence and Western blot analysis.The efficacy of CeO_(2)NPs was tested in an AT rat model in comparison with the control.Results:CeO_(2)NPs not only significantly scavenged multiple ROS and suppressed ROS-induced inflammatory reactions but also protected cell proliferation under oxidative stress induced by tert-butyl hydroperoxide(TBHP).Moreover,CeO_(2)NPs could promote NRF_(2)nuclear translocation for anti-oxidation and anti-inflammation through the ERK signaling pathway.In a rat model of collagenase-induced tendon injuries,CeO_(2)NPs showed significant therapeutic efficacy by ameliorating tendon damage.Conclusion:The present study provides valuable insights into the molecular mechanism of CeO_(2)NPs to ameliorate ROS in tenocytes via the ERK/NRF_(2)signaling pathway,which underscores the potential of CeO_(2)NPs for application in the treatment of enthesopathy healing.

关 键 词：CeO_(2)nanozymes enthesiopathy NRF2 translocation ERK signaling 

分 类 号：TB383[一般工业技术—材料科学与工程]