Turning carbon dots into selenium bearing nanoplatforms with in vitro GPx-like activity and pro-oxidant activity  

作　　者：Laura Perez-Garrido Mariano Ortega-Muñoz Fernando Hernandez-Mateo F.Javier Lopez-Jaramillo Francisco Santoyo-Gonzalez 

机构地区：[1]Department of Organic Chemistry,Biotechnology Institute,School of Sciences,Campus Fuentenueva sn,University of Granada,18071-Granada,Spain

出　　处：《Nano Research》2023年第5期7784-7791,共8页纳米研究（英文版）

基　　金：the Spanish institution Ministerio de Ciencia,Innovacion y Universidades(No.CTQ2017-86125-P).

摘　　要：Selenium(Se)has been defined as the“Janus element”,with one face showing antioxidant activity and the other pro-oxidant activity.The biological effect of Se depends on both dose and speciation.Se nanoparticles are attracting major interest,although their large-scale preparation for biomedical applications is not trivial.We hypothesize that acid anhydride-coated carbon dots(AACD)are an attractive platform for preparing nanoparticles containing chemically defined Se.The reaction of AA-CD with 3-selenocyanatopropan-1-amine yields carbon dots bearing selenocyanate and carboxylate groups(CD-SeCN)that allow for tuning the hydrosolubility.CD-SeCN has a Se content of 0.36μmol per mg of nanoparticles,they show the typical photoluminescence of carbon dots.The selenocyanate groups(SeCN)exhibited glutathione peroxidase-like activity and cytotoxicity.Data show that antioxidant behavior differs between normal and tumor cells,the evaluation on HEK293 and A549 cells reveals that the toxicity of CD-SeCN depends on dose,time,intracellular glutathione(GSH)content.The toxicity of CD-SeCN decreases with the time of incubation and the cell death mechanism switches from necrosis to apoptosis,indicating that CD-SeCN is neutralized.Additionally,high levels of intracellular GSH exert a protective effect.These results support a pharmacological potential in cancers with low levels of intracellular GSH.The use of AA-CD as nanoplatforms is a general strategy that paves the way for the engineering of advanced nanosystems.

关 键 词：selenium nanoparticles carbon dots glutathione peroxidase-like activity Janus element NANOMEDICINE 

分 类 号：TB383[一般工业技术—材料科学与工程]