柚皮苷减轻蛛网膜下腔出血大鼠早期脑损伤的研究  

Naringin alleviates early brain injury in rats with subarachnoid hemorrhage

作　　者：韩雨薇 王晨辰 梁国标 李晓明 Han Yuwei;Wang Chenchen;Liang Guobiao;Li Xiaoming(Department of Neurosurgery,General Hospital of Northern Theater Command of PLA,Shenyang 110016,China)

机构地区：[1]北部战区总医院神经外科,沈阳110016

出　　处：《国际生物医学工程杂志》2022年第6期479-484,共6页International Journal of Biomedical Engineering

摘　　要：目的探讨柚皮苷对蛛网膜下腔出血(SAH)大鼠早期脑损伤的影响及其可能的作用机制。方法将大鼠随机分为假手术组、模型组和柚皮苷组,每组8只,采用血管内穿孔建立大鼠SAH模型,0.5 h后模型组和柚皮苷组分别腹腔注射0.9%NaCl或柚皮苷40 mg/kg。对SAH评分、神经功能评分、脑水肿和血脑屏障通透性进行检测。酶联免疫吸附测定(ELISA)检测烟酰胺腺嘌呤二核苷酸(NAD+)和炎症因子水平,Western Blot检测聚(ADP-核糖)聚合酶-1(PARP-1)、凋亡诱导因子(AIF)和蛋白酶体活化受体(PAR)蛋白表达情况,实时荧光定量PCR(qRT-PCR)检测PARP-1 mRNA表达情况,免疫荧光检测神经元凋亡。结果与模型组相比,柚皮苷能改善神经功能(P<0.01),减少脑水肿和依文思蓝渗出(均P<0.01)。与模型组相比,柚皮苷能增加NAD+含量(P<0.001),明显降低白细胞介素(IL)-1β、IL-6和肿瘤坏死因子-α(TNF-α)的蛋白水平(均P<0.001),降低体内PARP-1/AIF通路相关蛋白的表达(均P<0.001)。此外,柚皮苷可抑制SAH后早期脑损伤中神经元细胞凋亡。结论柚皮苷可改善SAH后早期脑损伤,可能是通过抑制PARP-1/AIF通路实现的。Objective To investigate the effects of naringin on early brain injury in rats with subarachnoid hemorrhage and its possible mechanism of action.Methods Rats were randomly divided into the sham operation group,the model group,and the naringin group.Each group had 8 rats.The SAH model was established by intravascular perforation,and then rats in the model group and the naringin group were administered 0.9%NaCl or naringin 40 mg/kg by intraperitoneal injection after 0.5 h.SAH score,neurological function score,cerebral edema,and blood-brain barrier permeability were detected.The level of NAD+and nflammatory factors were detected by ELISA.The expression of poly(ADP-ribose)polymerase-1(PARP-1),apoptosis inducing factor(AIF),and protease-activated receptor(PAR)proteins was detected by Western Blot.The expression of PARP-1 mRNA was detected by quantitative real-time fluorescence PCR(qRT-PCR).Neuronal apoptosis was detected by an immunofluorescence assay.Results Compared with the model group,naringin treatment improved neurological function(P<0.01),reduced cerebral edema and Evans blue exudation(all P<0.01),increased the content of NAD+(P<0.001),reduced IL-1β,IL-6 and TNF-αlevels(all P<0.001),and reduced the expression of PARP-1/AIF pathway-related proteins in vivo(all P<0.001).In addition,naringin could inhibit neuronal apoptosis in early brain injury after SAH.Conclusions Naringin can improve the early brain injury after SAH,which may be achieved by inhibiting the PARP-1/AIF pathway.

关 键 词：柚皮苷 蛛网膜下腔出血 聚(ADP-核糖)聚合酶-1 凋亡诱导因子 蛋白酶体活化受体 

分 类 号：R285.5[医药卫生—中药学]