脱落酸对NaCl胁迫下红砂种子萌发的影响  

Effects of abscisic acid on Reaumuria soongorica seed germination under NaCl stress

作　　者：马强 苏世平[1] 李毅[1] 张䶮 师微柠 席杰 MA Qiang;SU Shiping;LI Yi;ZHANG Yan;SHI Weining;XI Jie(College of Forestry,Gansu Agricultural University,Lanzhou 730070,Gansu,China;Forestry and Grassland Service Center of Tongwei County,Dingxi 743300,Gansu,China)

机构地区：[1]甘肃农业大学林学院,甘肃兰州730070 [2]通渭县林业和草原服务中心,甘肃定西743300

出　　处：《草业科学》2023年第4期925-934,共10页Pratacultural Science

基　　金：国家自然科学基金(32060335);对发展中国家常规性科技援助项目(KY202002011);甘肃省自然科学基金(21JR7RA814)。

摘　　要：为揭示脱落酸(ABA)处理对NaCl胁迫下红砂(Reaumuria soongorica)种子萌发特性的影响,本研究采用双因素正交试验,研究不同ABA浓度(0、10、20、30、40、50 mg·L^(-1))和NaCl浓度(0、25、50、75、100、150 mmol·L^(-1))处理下红砂种子萌发特性的变化。结果表明:ABA和NaCl处理对红砂种子的发芽率、发芽势、活力指数、发芽指数、胚芽长和胚根长均有极显著影响(P<0.01),两者交互(ABA×NaCl)对发芽势有极显著影响(P<0.01),对发芽率有显著影响(P<0.05)。在0~150 mmol·L^(-1) NaCl胁迫下,以20 mg·L^(-1) ABA处理作用效应最大,可显著促进红砂种子的萌发。与0 mg·L^(-1) ABA、25 mmol·L^(-1) NaCl处理相比,20 mg·L^(-1) ABA、25 mmol·L^(-1) NaCl处理下红砂种子发芽率、发芽势、活力指数、发芽指数显著升高了20.33%、31.25%、63.95%和31.20%,胚芽以及胚根长度显著升高了24.53%和41.98%。因此,在今后荒漠区红砂种群人工辅助更新中建议采用20 mg·L^(-1) ABA浸种24 h后进行直播造林,可有效缓解NaCl胁迫对红砂种子萌发以及幼苗生长造成的伤害。We explored the effects of abscisic acid(ABA)and NaCl stress on the germination characteristics of Reaumuria soongarica seeds using two-factor orthogonal experiments.Different ABA(0,10,20,30,40,and 50 mg·L^(-1))and NaCl(0,25,50,75 and 150 mmol·L^(-1))concentrations were used.ABA and NaCl treatment had an considerable significant influence(P<0.01)on germination rate and potential,vigor and germination index,and germ and root length.Furthermore,germination potential and rate were significantly affected by the interaction of ABA and NaCl(P<0.01 and P<0.05,respectively).NaCl(0~150 mmol·L^(-1))stress and ABA(20 mg·L^(-1))treament had the greatest effect on accelerating R.soongarica seed growth.Compared with those under 0 mg·L^(-1) ABA and 25 mmol·L^(-1) NaCl treatment,seeds under 20 mg·L^(-1) ABA and 25 mmol·L^(-1) NaCl treatment exhibited an increase in germination rate and potential,vigor and germination index,and germ and root length by 20.33%,31.25%,63.95%,31.20%,24.53%,and 41.98%,respectively.Hence,we advised that R.soongarica seeds should be soaked in 20 mg·L^(-1) ABA in the further artificial auxiliary regeneration for 24 h before direct seeding for afforestation in desert areas,which presents an effective method of alleviating the damage caused by seed germination and seedling growth of R.soongarica seeds under NaCl treatment.

关 键 词：红砂 脱落酸 NACL胁迫 发芽率 发芽势 活力指数 响应机制 

分 类 号：S793.9[农业科学—林木遗传育种]