Insecticidal Potential of α-Pinene and β-Caryophyllene against Myzus persicae and Their Impacts on Gene Expression  

作　　者：Talha Ali Chohan Tahir Ali Chohan Muhammad Zahid Mumtaz Muhammad Waqar Alam Salah ud Din Iqra Naseer Ayesha Riaz Tayyeba Naseem Areeba Iftikhar Dur ENajaf Ali Mubashir Hassan Hayssam M.Ali 

机构地区：[1]Institute of Molecular Biology and Biotechnology(IMBB),The University of Lahore,Lahore,54000,Pakistan [2]Institute of Pharmaceutical Sciences,University of Veterinary and Animal Sciences,Lahore,54000,Pakistan [3]Deparment of Plant Pathology,University of Okara,Okara,56300,Pakistan [4]Deparment of Bioinformatics,University of Okara,Okara,56300,Pakistan [5]Department of Soil Science,The Islamia University of Bahawalpur,Bahawalpur,63100,Pakistan [6]Department of Zoology,The Islamia University of Bahawalpur,Bahawalpur(Bahawalnagar Campus),63100,Pakistan [7]The Steve and Cindy Rasmussen Institute for Genomic Medicine,Nationwide Children’s Hospital,Columbus,Ohio,43205,USA [8]Department of Botany and Microbiology,College of Science,King Saud University,Riyadh,11451,Saudi Arabia

出　　处：《Phyton-International Journal of Experimental Botany》2023年第7期1943-1954,共12页国际实验植物学杂志（英文）

基　　金：funded by the Researchers Supporting Project Number(RSP2023R123),King Saud University,Riyadh,Saudi Arabia.

摘　　要：Myzus persicae(M.persicae)is now considered a threat to agricultural crops due to economic losses.Numerous synthetic insecticides applied every year against M.persicae,are reported to be unsafe for environment,humans,and beneficial insects.Furthermore,several species of Myzus have been found to develop resistance due to over application of these insecticides.Therefore,it is required to find some novel insecticide that would be safe for the environment as well as for humans.In the current study,two major pure constituentsα-pinene andβ-caryophyllene were evaluated for their insecticidal potential against M.persicae using a fumigant toxicity assay.Furthermore,impact ofα-pinene andβ-caryophyllene on expression of five different genes,e.g.,HSP 60,FPPS I,OSD,TOL and ANT responsible for reproduction,dispersion,and growth of M.persicae has also been investigated.To perform fumigant toxicity assay,five different concentrations(3.5,4,4.5,5 and 6μL L−1)ofα-pinene andβ-caryophyllene were prepared.Lethal concentration(LC)was calculated,and gene expression studies were executed through qRT PCR at LC30 ofα-pinene andβ-caryophyllene.Both constituents demonstrated excellent fumigant toxicity effects against M.persicae at all five concentrations.However,α-pinene shows significantly better results(98%)as compared toβ-caryophyllene(80%)after 72 h at 6μL L−1 of dose.The highest upregulation in expression was demonstrated at LC30 dose ofα-pinene in five in three out of five genes understudy(TOL,ANT,and FPPS I).Conversely,two genes HSP 60 and OSD demonstrated downregulation at LC30 dose ofβ-caryophyllene.Conclusively,our results highlighted the promising insecticidal potential of both compoundsα-pinene andβ-caryophylleneby interfering with the reproduction and development related processes in M.persicae,allowing us to recommend the phytoconstituents under investigation as an ecofriendly alternative to synthetic insecticides.

关 键 词：FUMIGATION M.persicae gene expression real time PCR Α-PINENE β-caryophyllene 

分 类 号：Q94[生物学—植物学]