Analysis of Subcellular Localization and Pathogenicity of Plum Bark Necrosis Stem-Pitting Associated Virus Protein P6  

作　　者：Yuanyuan Li Jinze Mu Qingliang Li Huabing Liu Xuefeng Yuan Deya Wang 

机构地区：[1]College of Life Sciences,Zaozhuang University,Zaozhuang Cherry Virus Disease Rapid Diagnosis and Green Control Technology Innovation Center,Zaozhuang,277160,China [2]College of Plant Protection,Shandong Agricultural University,Shandong Agricultural Microbiology Key Laboratory,Tai’an,271018,China [3]Yicheng Agricultural and Rural Comprehensive Service Center,Zaozhuang,277300,China

出　　处：《Phyton-International Journal of Experimental Botany》2023年第7期2079-2085,共7页国际实验植物学杂志（英文）

基　　金：funded by the National Natural Science Foundation of China(32102143);Shandong Province Natural Sciences Foundation of China(ZR2019PC011 and ZR2020QC122);Scientific Research Foundation for Ph.D.Programs of Zaozhuang University(2018BS040 and 2018BS042);Science and Technology Program of Zaozhuang(2019NS03).

摘　　要：Infection of plum bark necrosis stem pitting associated virus(PBNSPaV)has been reported in many Prunus species in several countries,causing significant economic losses.The very small proteins encoded by plant viruses are often overlooked due to their short sequences and uncertain significance.However,numerous studies have indicated that they might play important roles in the pathogenesis of virus infection.The role of small hydrophobic protein P6,encoded by the open reading frame 2 of PBNSPaV,has not been well explored.In this study,we amplified the P6 fragment from a PBNSPaV isolate by RT-PCR using specific primers and found that it is 174 bp long and encodes a protein of approximately 6.3 kD with a transmembrane domain.Subcellular localization analysis of P6 proteins in tobacco leaves showed that P6 localizes to the cytomembrane and nuclear membrane.To further clarify the pathogenicity of P6 proteins,we constructed a PVX-P6 expression vector by inserting the p6 fragment into a potato virus X(PVX)-based vector and transformed it into Agrobacterium tumefaciens GV3101.Infiltration of Nicotiana benthamiana(N.benthamiana)with the PVX vector-transformed A.tumefaciens led to slight mosaic symptoms at 14 days of post-inoculation.Meanwhile,infiltration with the PVX-P6 vector-transformed A.tumefaciens resulted in no significant symptoms.These results demonstrated that heterologous expression of P6 in N.benthamiana could not enhance the pathogenicity of PVX.Our study indicates that P6 may not be a potential pathogenic factor associate with the causing of symptoms,and the mode of action of PBNSPaV-P6 protein remains to be further studied.

关 键 词：Plum bark necrosis stem-pitting associated virus P6 protein subcellular localization PATHOGENICITY 

分 类 号：Q94[生物学—植物学]