Th17和Treg细胞与慢性光化性皮炎的相关性  

Clinical Correletions between Helper T Cells 17,Regulatory T Cells and Chronic Actinic Dermatitis

作　　者：杨成林 胡婕 王静 王敏华 黄玲 YANG Chenglin;HU Jie;WANG Jing;WANG Minhua;HUANG Ling(Dept.of Internal Medicine,Dali Nursing Vocational College,Dali Yunnan,671000;Dept.of Dermatology,Traditional Chinese Hospital of LuAn,Luan Anhui 237000;Dept.of Dermatology,Jinshan Hospital of Fudan University,Shanghai 200540;Dept.of Dermatology,The 1st Affiliated Hospital of Dali University,Dali Yunnan 671001,China)

机构地区：[1]大理护理职业学院内科教研组,云南大理671000 [2]六安市中医院皮肤科,安徽六安237000 [3]复旦大学附属金山医院皮肤科,上海200540 [4]大理大学第一附属医院皮肤科,云南大理671001

出　　处：《昆明医科大学学报》2023年第5期95-101,共7页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助项目(2015Y382)。

摘　　要：目的探讨外周血中辅助性T淋巴细胞17(Th17 cells)和调节性T淋巴细胞(Treg cells)在慢性光化性皮炎(chronic actinic dermatitis,CAD)发病机制中可能的作用。方法流式细胞术检测25例CAD患者及20名健康志愿者外周血CD4+T淋巴细胞中Th17细胞/Tregd的比值,同时应用免疫组织化学法检测25例CAD患者和10例健康人皮肤组织皮损白介素-17(IL-17)、叉头转录因子p3(Foxp3)的表达。结果CAD患者Th17明显高于志愿者;CAD患者Treg细胞明显少于志愿者(P<0.05);CAD患者的Th17/Treg显著高于健康志愿者(P<0.05)。Th17细胞和EASI评分2者间存在正相关关系(P<0.05);Treg细胞和EASI评分间存在负相关关系;CAD组患者皮损中IL-17高表达,与健康对照组相比,差异有统计学意义(P<0.05),Foxp3在皮肤中的表达与健康对照组相比差异无统计学意义(P>0.05)。结论CAD患者存在Th17/Treg的失衡,由此导致的免疫反应向促炎方向发展,可能是CAD重要的发病机制。Objective To investigate the the possible roles of helper T cells 17(Thl7 cells)and regulatory T cells(Treg cells)of chronic actinic dermatitis.Methods The percentages of Th17 cells and Treg cells in CD4+T cells in peripheral blood of 25 CAD patients and 20 healthy volunteers were detected by flow cytometry,and the expression of IL-17 and Foxp3 in skin lesions of 25 patients with chronic actinic dermatitis and 10 healthy people were detected by immunohistochemistry.Results Compared with the healthy volunteers,Th17 cells in CAD patients were significantly more than those in volunteers;Treg cells in CAD patients were significantly less than those in volunteers;Th17/Treg in CAD patients was significantly higher than that in volunteers and all the differences were statistically significant(P<0.05).The correlation coefficients of Th17 cells and EASI scores showed that there was a positive correlation between them(P<0.05);the correlation coefficients of Treg cells and EASI scores showed that there was a negative correlation between them.The expression of IL-17 in lesions of CAD group was higher than that of healthy control group and the difference was statistically significant(P<0.05).There was no significant difference in the expression of Foxp3 in the skin compared with the healthy control group(P>0.05).Conclusion The imbalance of Th17/Treg in patients with CAD leads to the development of immune response to pro-inflammatory,which may be an important pathogenesis of CAD.

关 键 词：慢性光化性皮炎 辅助性T淋巴细胞17 调节性T淋巴细胞 

分 类 号：R75[医药卫生—皮肤病学与性病学]