健康献血者机采血小板储存中LncRNA LIPCAR的表达及初步研究  

Expression and Preliminary Study of LncRNA LIPCAR in Platelet Storage of Mechanically Collected Platelets from Healthy Blood Donors

作　　者：张文娟[1] 郭逸[1] 段勇[1] 冯娜[1] 彭鹏[1] 景媛媛[1] ZHANG Wen-juan;GUO Yi;DUAN Yong;FENG Na;PENG Peng;JING Yuan-yuan(Shaanxi Blood Center,Xi’an 710061,China)

机构地区：[1]陕西省血液中心,西安710061

出　　处：《现代检验医学杂志》2023年第3期119-122,158,共5页Journal of Modern Laboratory Medicine

基　　金：西安市科技计划项目(22YXYJ0050):长链非编码RNA在机采血小板储存损伤中的作用及分子机制。

摘　　要：目的探讨健康献血者机采血小板伴随储存时间延长长链非编码RNA(long non-coding RNA,LncRNA)中预测心脏重塑的基因间长链非编码RNA(long intergenic noncoding RNA predicting cardiac remodeling,LIPCAR)的表达变化及意义。方法收集陕西省血液中心2021年11月~2022年7月正常捐献者机采血小板32份,于血小板专用22±2℃恒温振荡保存箱内保存,针对同一机采血小板样本通过延长储存时间(1,3,5,7和9天)检测血小板质量,使用实时荧光定量PCR(real-time quantitative PCR,qRT-PCR)检测LIPCAR在不同储存时间的表达量,并分析其表达水平变化与捐献者性别和血型的关联性。结果经检测血小板质量合格,在储存的第1,3,5,7和9天,伴随储存时间延长LIPCAR表达量逐渐增高,数据总体差异有统计学意义(H=89.46,P<0.001)。对不同天数之间进行两两多重比较,第1天和第5,7,9天,第3天和第7,9天校正后的P值均小于0.001;第5天和第9天校正后的P=0.016,差异均有统计学意义。不同储存天数LIPCAR表达量根据性别分析,发现在第5天和第1天(t=2.189,P=0.038)、第7天和第1天(t=2.320,P=0.028)、第9天和第1天(t=2.264,P=0.032)男性表达量高于女性,差异具有统计学意义;不同血型之间总体存在同质性差异(F=3.160,P=0.043),差异具有统计学意义。结论经检测合格用于临床输血的机采血小板随着储存时间延长,第5,7和9天样本中LIPCAR含量比第1天明显增高,LIPCAR对血小板储存条件敏感,有可能成为血小板储存损伤(platelet storage lesion,PSL)潜在的生物学标志物。Objective To investigate the expression changes and significance of long intergenic noncoding RNA predicting cardiac remodeling(LIPCAR)in the long-chain non-coding RNA(LncRNA)along with time expand of normal mechanically collected platelets.Methods A total of 32 normal mechanically collected platelets from normal donors from Shaanxi Blood Center from November 2021 to July 2022 were collected and stored in a special 22±2℃thermostatic oscillation box for platelets.The quality of platelets was detected by prolonging the storage time(1,3,5,7 and 9 days).The expression levels of LIPCAR at different storage times of platelet samples were detected by qRT-PCR.The correlation between the change of expression level with the sex and blood type of the donor were analyzed.Results The quality of that detect platelets is qualify.On 1,3,5,7 and 9 day of storage,the expression of LIPCAR increased gradually along with the prolongation of storage days,and the overall data showed statistical significance difference(H=89.46,P<0.001).Pairwise multiple comparisons were made between days,P-values corrected were less than 0.001 on days 1 and 5,1 and 7,1 and 9,3 and 7,3 and 9.P=0.016 after correction on the 5 and 9 days,and the differences were statistically significant.According to gender analysis,the expression of LIPCAR on days 5th and 1th(t=2.189,P=0.038),7 and 1(t=2.320,P=0.028),9th and 1th(t=2264,P=0.032)showed statistical differences on different storage days.There was overall homogeneity difference between the different blood groups(F=3.160,P=0.043),with statistical significance.Conclusion The normal mechanically collected platelets with the storage time prolonging,the LIPCAR content in the samples on the 5th,7th and 9th day was significantly higher than that on the 1st day.LIPCAR was sensitive to platelet storage conditions,and might become a potential biomarker of PSL.

关 键 词：机采血小板 长链非编码RNA 预测心脏重塑的基因间长链非编码RNA 血小板储存损伤 储存时间 

分 类 号：R457.12[医药卫生—治疗学]