PCSK9基因多态性及其血清水平与维持性血液透析患者颈动脉粥样硬化的关系  

Associations of PCSK9 gene polymorphisms and its serum level with carotid atherosclerosis in maintenance hemodialysis patients

在线阅读下载全文

作  者:崔俊[1] 亢宁苏 陆建勋[1] 郑露[1] CUI Jun;KANG Ning-su;LU Jian-xun;ZHENG Lu(Department of Nephrology,Nantong Third People's Hospital,Nantong 226000,Jiangsu,China)

机构地区:[1]南通市第三人民医院肾内科,江苏南通226000

出  处:《广东医学》2023年第4期503-508,共6页Guangdong Medical Journal

基  金:南通市卫生健康委员会科研课题(MB2020035)。

摘  要:目的探讨前蛋白转化酶枯草杆菌蛋白酶/Kexin 9型(PCSK9)基因单核苷酸多态性(SNPs)及其血清水平与维持性血液透析(MHD)患者颈动脉粥样硬化(CAS)的关系.方法选取2021年1月至2022年5月南通市第三人民医院肾内科收治的220例MHD患者,根据是否发生CAS分为CAS组(81例)和非CAS组(139例).采用酶联免疫吸附法检测血清PCSK9水平,限制性内切酶法检测PCSK9基因rs11206510、rs562556、rs505151、rs2479409位点SNPs.采用多因素logistic回归分析血清PCSK9水平与MHD患者CAS的关系,受试者工作特征(ROC)曲线分析血清PCSK9水平对MHD患者CAS的预测价值.结果CAS组PC-SK9基因rs11206510位点T等位基因频率高于非CAS组,rs505151位点G等位基因频率高于非CAS组(P<0.05).两组患者PCSK9基因rs562556、rs2479409位点基因型频率和等位基因频率比较差异无统计学意义(P>0.05).CAS组血清PCSK9水平高于非CAS组(P<0.05).多因素logistic回归分析显示,PCSK9升高(OR=1.022,95%CI:1.014~1.029)为MHD患者CAS的独立危险因素(P<0.05).ROC曲线分析显示,血清PCSK9水平为247.38 ng/mL时,预测MHD患者CAS的曲线下面积、敏感度、特异度分别为0.804、72.84%、78.42%.结论PCSK9基因rs11206510、rs505151位点SNPs和血清PCSK9水平与MHD患者CAS相关,血清PCSK9水平可作为MHD患者CAS的辅助预测指标.Objective To investigate the correlations of the single nucleotide polymorphism(SNP)of the proprotein convertase subtilisin/Kexin type 9(PCSK9)gene and its serum level with carotid atherosclerosis(CAS)in maintenance hemodialysis(MHD)patients.Methodss From January 2021 to May 2022,220 patients with MHD admited to the Department of Nephrology of the Third People's Hospital of Nantong,Jiangsu Province were selected and divided into CAS group(n=81)and non-CAS group(n=139)according to whether they had carotid atherosclerosis.Serum PCSK9 levels were measured by enzyme-linked immunosorbent assay,and PCSK9 gene rs11206510,rs562556,rs505151,rs2479409 locus SNPs were assessed by restriction endonuclease assay.Multi-factor logistic regression was used to analyze the correlation between serum PCSK9 level and CAS in MHD patients;and ROC curve were used to analyze the predictive value of serum PCSK9 level on CAS in MHD patients.Results The frequency of the T allele at locus rs11206510 was significantly higher in the CAS group than in the non-CAS group,and the frequency of the G allele at locus rs505151 was significantly higher than in the non-CAS group(P<0.05).There was no significant difference in genotype frequencies or allele frequencies at the rs562556 or rs2479409 loci of the PCSK9 gene between the two groups(P>0.05).Serum PCSK9 levels were significantly higher in the CAS group than in the non-CAS group(P<0.05).Multi-factor logistic regression analysis showed that elevated PCSK9(OR=1.022,95%CI:1.014-1.029)was an independent risk factor for CAS in patients with MHD(P<0.05).ROC curve analysis showed that the area under the curve,sensitivity and specificity for predicting CAS in MHD patients at a serum PCSK9 level of 247.38 ng/mL were 0.804,72.84%and 78.42%respectively.Conclusion The PCSK9 gene rsl1206510,rs505151 locus SNPs and serum PCSK9 levels are associated with CAS in MHD patients,and serum PCSK9 levels can be used as an auxiliary predictor of CAS in MHD patients.

关 键 词:维持性血液透析 颈动脉粥样硬化 前蛋白转化酶枯草杆菌蛋白酶/Kexin9型 单核苷酸多态性 

分 类 号:R692.5[医药卫生—泌尿科学] R459.5[医药卫生—外科学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象