组学技术在重离子辐射微生物诱变育种中的应用  

Application of Omics Techniques in Incluced Breecling via Heavy Ion Beam Irradiating Microorganisms

作　　者：雷彩荣 郭晓鹏 柴冉 张苗苗[1,2,4] 任军乐 陆栋 LEI Cai-rong;GUO Xiao-peng;CHAI Ran;ZHANG Miao-miao;REN Jun-le;LU Dong(Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000;University of Chinese Academy of Sciences,Beijing 100049;Lanzhou University of Technology,Lanzhou 730050;Key Laboratory of Microbial Resources Exploitation and Application,Lanzhou 730070)

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院大学,北京100049 [3]兰州理工大学,兰州730050 [4]甘肃省微生物资源开发利用重点实验室,兰州730070

出　　处：《生物技术通报》2023年第5期54-62,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(11905265,11975284);甘肃省自然科学基金项目(20JR5RA552);中国科学院“西部青年学者”(E023211Y)。

摘　　要：重离子辐射诱变具有诱变率高、诱变谱宽、突变体易稳定等优势,在微生物育种实践中已得到广泛应用。随着测序技术的发展,对重离子辐射诱变效应的研究可以实现较为全面地了解突变体在基因组、转录组、蛋白质组和代谢组等多个层面的生物学信息。本文综述了利用重离子辐射诱变技术进行微生物育种的研究进展,以及联合高通量测序技术探究重离子诱变产生的生物学效应机理。在此基础上,进一步探讨利用组学方法研究重离子诱变微生物的新思路,旨为重离子诱变微生物育种技术提供参考和建议。Heavy ion beam(HIB)irradiation,with high mutagenesis rate,broad mutagenesis spectrum and stable mutant,has been widely used in the implementation of industrial microbial breeding.With the development of sequencing technology,a more comprehensive understanding of the biological information at multiple levels such as genome,transcriptome,proteome,and metabolome may be achieved via study on the mutagenesis effect of HIB.This paper summarizes the research progress of microbial breeding using HIB,combining highthroughput sequencing technology to explore the biological effects of heavy ion mutagenesis.On this basis,a new idea of using omics methods to study the mutagenesis effect of HIB was further discussed with aim to provide references and suggestions for the breeding technology.

关 键 词：重离子辐射 微生物诱变育种 多组学 

分 类 号：S51[农业科学—作物学]