牛诱导多能干细胞的建立及应用研究进展  

Research Progress in the Application and Establishment of Bovine Induced Pluripotent Stem Cells

作　　者：曾虹 曾睿琳 付伟 吉文汇[1] 兰道亮 ZENG Hong;ZENG Rui-lin;FU Wei;JI Wen-hui;LAN Dao-liang(School of Animal&Veterinary Sciences,Southwest Minzu University,Chengdu 610041;Ministry of Education of Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource and Utilization,Chengdu 610041;Sichuan Province of Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource and Utilization,Chengdu 610041)

机构地区：[1]西南民族大学畜牧兽医学院,成都610041 [2]青藏高原动物遗传资源保护与利用教育部重点实验室,成都610041 [3]青藏高原动物遗传资源保护与利用四川省重点实验室,成都610041

出　　处：《生物技术通报》2023年第5期130-141,共12页Biotechnology Bulletin

基　　金：国家重点研发计划项目(2021YFD1600200);西南民族大学中央高校基本科研业务费专项资金资助(2021NYYXS94)。

摘　　要：诱导性多能干细胞(induced pluripotent stem cells,iPSCs)是指通过导入特定的转录因子将终末分化的体细胞重编程为可以无限增殖更新并具有分化为三胚层多种细胞类型的一类干细胞系。目前对人与小鼠的iPSCs研究已经取得了很多重要成果,但其他动物,如牛等经济型有蹄类家畜iPSCs的研究始终没有突破性的进展。如何将外源转录因子通过重编程载体高效安全地导入体细胞中并持续表达是生产牛诱导多能干细胞(bovine induced pluripotent stem cells,biPSCs)的主要瓶颈。本文就biPSCs建立中重编程系统的选择、诱导因子的选择、小分子化合物的添加等方面进行综述,以期为进一步完善biPSCs及牛胚胎干细胞系的建立提供参考。Induce pluripotent stem cells(iPSCs)refer to a class of stem cell lines that reprogram terminally differentiated somatic cells into infinite proliferation and regeneration and have multiple cell types differentiated into three germ layers by introducing specific transcription factors.At present,many important achievements have been made in the research of iPSCs in humans and mice,but no breakthrough has been made in the research of iPSCs in other animals,such as cattle and other economic ungulates.How to efficiently and safely introduce foreign transcription factors into somatic cells through reprogramming vectors and continuously express them is the main bottleneck in the production of bovine induced pluripotent stem cells.This article reviews the selection of reprogramming system,induction factors and small molecule compounds in the establishment of bovine iPSCs,aiming to provide reference for further improvement of biPSCs and the establishment of bovine embryonic stem cell lines.

关 键 词：牛诱导多能干细胞 体细胞重编程 培养体系 小分子化合物 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]