沙福芽孢杆菌GX-H6的分离鉴定及对水稻细菌性条斑病的防病效果  

Isolation and Identification of a Bacillus safensis Strain GX-H6 and Its Biocontrol Effect on Bacterial Leaf Streak of Rice

作　　者：任沛东 彭健玲 刘圣航 姚姿婷 朱桂宁[1,3,4] 陆光涛 李瑞芳[1,3,4] REN Pei-dong;PENG Jian-ling;LIU Sheng-hang;YAO Zi-ting;ZHU Gui-ning;LU Guang-tao;LI Rui-fang(Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530017;College of Life Science and Technology,Guangxi University,Nanning 530005;Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs,Nanning 530017;Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning 530017)

机构地区：[1]广西农业科学院植物保护研究所,南宁530017 [2]广西大学生命科学与技术学院,南宁530005 [3]农业农村部华南果蔬绿色防控重点实验室,南宁530017 [4]广西作物病虫害生物学重点实验室,南宁530017

出　　处：《生物技术通报》2023年第5期243-253,共11页Biotechnology Bulletin

基　　金：国家自然科学基金项目(32160617);广西农业科学院科技发展基金(桂农科2021JM13,桂农科2021ZX23,桂农科2021YT063)。

摘　　要：水稻细菌性条斑病(bacterial leaf streak,BLS)由稻黄单胞菌稻生致病变种(Xanthomonas oryzae pv.oryzicola,Xoc)侵染引起,已成为我国南方水稻种植区的一个重要病害。为了筛选防治BLS的生防菌,以Xoc野生型菌株GX01为指示菌,采用含菌平板稀释法和牛津杯法,从花生根际土壤中筛选到一株对Xoc具有拮抗作用的细菌,编号为GX-H6。根据形态学观察、生理生化特征以及16S rDNA和进化树分析,鉴定该菌株属于沙福芽孢杆菌(Bacillus safensis)。拮抗实验表明,B.safensis GX-H6菌株能够对多种黄单胞菌以及植物病原真菌具有较好的拮抗活性,尤其对引起水稻发生白叶枯病(bacterial blight,BB)的稻黄单胞菌稻致病变种(Xanthomonas oryzae pv.oryzae,Xoo)的拮抗效果最显著。温室和田间水稻植株试验表明,GX-H6菌株能较好地防治BLS和BB。对GX-H6菌株的基因组进行分析,发现该菌株的基因组中拥有与抗真菌、环境竞争相关的基因,同时也拥有地衣杆菌素(lichenysin)、植物阿唑霉素(plantazolicin)和溶杆菌素(bacilysin)合成相关基因。这为生产应用提供了新的微生物资源,以及为后续的抑菌机理研究提供了新材料。Bacterial leaf streak(BLS),caused by Xanthomonas oryzae pv.oryzicola(Xoc),is emerging as a serious disease in rice fields of southern China.In order to screen biocontrol bacteria for controlling BLS,we had Xoc wild-type strain GX01 as indicator,a bacterium with antagonistic effect to Xoc was isolated from the samples of peanut rhizosphere soil by using bacterial plate dilution and Oxford cup methods,and named as GX-H6.Based on the morphological,physiological and biochemical data,16S rDNA and evolutionary tree analysis,this strain was identified to be Bacillus safensis.Antagonistic experiments revealed that the B.safensis GX-H6 strain effectively repressed the growths of several xanthomonads and plant pathogenic fungi,particularly the bacterium Xanthomonas oryzae pv.oryzae,the causal agent of bacterial blight(BB)of rice.Further greenhouse and field rice plant experiments showed that this strain effectively controlled BLS and BB of rice.Genome analysis indicated that the GX-H6 strain contained a variety of genes related to antifungal,environmental competition,and the production of lichenysin,plantazolicin and bacilysin.Overall,the B.safensis strain GX-H6 is a potential novel biocontrol agent for developing pesticides and exploring novel bacteriostatic mechanism in future.

关 键 词：水稻条斑病菌 黄单胞菌 沙福芽孢杆菌 拮抗 生物防治 

分 类 号：S43[农业科学—农业昆虫与害虫防治]