基于LC-QTOF-MS代谢组学解析牛樟芝子实体和菌丝体中氨基酸代谢差异  

Analysis of Amino Acid Metabolism Difference Between Fruiting Body and Mycelium of Taiwanofungus camphoratus by LC-QTOF-MS Metabonomics

作　　者：郑焕 林冬梅[1] 刘峻源 张引莲 林标声[3] 林占熺[1] 李晶[1] ZHENG Huan;LIN Dong-mei;LIU Jun-yuan;ZHANG Yin-lian;LIN Biao-sheng;LIN Zhan-xi;LI Jing(JUNCAO Sciences and Technology College of Fujian Agriculture and Forestry University,National Engineering Research Center of JUNCAO Technology,Fuzhou 350002;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002;College of Life Sciences,Longyan University,Longyan 364012)

机构地区：[1]福建农林大学菌草科学与技术研究院国家菌草工程技术研究中心,福州350002 [2]福建农林大学生命科学学院,福州350002 [3]龙岩学院生命科学学院,龙岩364012

出　　处：《生物技术通报》2023年第5期254-266,共13页Biotechnology Bulletin

基　　金：福建省科技厅自然科学基金(2021J01135);福建省科技厅重大专项(2021NZ029009);福建农林大学学科交叉融合专项“学科交叉融合推动菌草科学及产业高质量发展”(XKJC-712021030)。

摘　　要：为分析氨基酸在牛樟芝子实体和菌丝体代谢中的差异,采用LC-QTOF-MS系统质谱技术对牛樟芝子实体和菌丝体等3组样品进行代谢组分析,运用SIMCA软件(V16.0.2)对差异氨基酸代谢物进行筛选,分析重要氨基酸及其代谢途径,挖掘潜在标记物用于牛樟芝品种多样性和差异性分析。样品间主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)表明样品间在负离子条件下具有显著差异,在t检验P<0.05,VIP>1的条件下,牛樟芝子实体(ACFB)与液体培养14 d菌丝体(ACM-14)中有288个显著差异代谢物,其中主要差异氨基酸有15种;在ACFB与液体培养28 d菌丝体(ACM-28)中有332个显著差异代谢物,其中主要差异氨基酸有11种;ACM-14与ACM-28间存在283个显著差异代谢物,其中主要差异氨基酸有14种。牛樟芝子实体与菌丝体间氨基酸代谢差异大于菌丝体间,其中以L-精氨酸差异尤为显著。本研究为挖掘牛樟芝子实体、菌丝体氨基酸标记性代谢物和开发产品等提供理论参考。To analyze the amino acid differences in the metabolism of the fruiting body and mycelium of Taiwanofungus camphoratus,the LC-QTOF-MS system spectrometry was used to analyze the metabolome of three groups of samples,including the fruiting body and mycelium of T.camphoratus,and the SIMCA software(v16.0.2)was used to screen and identify the main amino acid metabolites,seek important differential metabolites,analyze important metabolic pathways,and tap potential markers for diversity analysis.Principal component analysis(PCA)and orthogonal projections to latent structures-discriminant analysis(OPLS-DA)among the samples demonstrated that there were differences among samples under negative ion conditions.Under the condition of t-test P<0.05 and VIP>1,288 metabolites were significantly different between the fruiting body(ACFB)and the mycelium cultured in liquid for 14 d(ACM-14),15 of them were the main amino acids.There were 332 significantly different metabolites between in ACFB and the mycelium cultured in liquid for 28 d(ACM-28),11 of them were the main amino acids.There were 283 significantly different metabolites between ACM-14 and ACM-28,of which 14 were the main amino acids.The difference of amino acid metabolism between the fruiting body and mycelia of Taiwanofungus camphoratus was greater than that between the mycelia,especially the difference of L-arginine.All above results may provide a theoretical basis for studying the differences of amino acid metabolism in fruiting body and mycelium of T.camphoratus,mining potential marker metabolites and developing products.

关 键 词：牛樟芝 LC-QTOF-MS 子实体 菌丝体 氨基酸代谢 

分 类 号：S64[农业科学—蔬菜学]