花生根部结瘤性状QTL定位  

Identification of major QTLs for nodule formation in peanut

作　　者：黄莉[1] 陈伟刚[1] 李威涛 喻博伦 郭建斌[1] 周小静[1] 罗怀勇[1] 刘念[1] 雷永[1] 廖伯寿[1] 姜慧芳[1] HUANG Li;CHEN Wei-Gang;LI Wei-Tao;YU Bo-Lun;GUO Jian-Bin;ZHOU Xiao-Jing;LUO Huai-Yong;LIU Nian;LEI Yong;LIAO Bo-Shou;JIANG Hui-Fang(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,Hubei,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《作物学报》2023年第8期2097-2104,共8页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13);中国农业科学院科技创新工程项目(2022-2060299-089-031)资助。

摘　　要：花生是我国重要的豆科油料作物和经济作物。根瘤是花生共生固氮的重要场所,研究花生根瘤形成的遗传基础,有助于更好地研究花生根瘤固氮能力和固氮特性。然而,关于花生根瘤形成的研究较少,调控花生根瘤形成的遗传机制不清楚。本研究通过对一个高世代RIL群体的根部结瘤性状进行调查,鉴定到7份根部不结瘤家系,根部不结瘤家系的叶绿素含量,以及株高、单株鲜重、单株干重均显著低于双亲。利用前期构建的SSR标记遗传连锁图,在A08和B07染色体上各鉴定到1个主效QTLqPNA08和qPNB07。通过InDel标记加密,将QTLqPNA08的区间由4.7 Mb缩小至1.6 Mb,遗传变异解释率由9.1%增加至16.4%;qPNB07的区间由9.9 Mb缩小至1.8 Mb,遗传变异解释率由7.1%增加至9.9%。根据基因功能注释,2个QTL区间分别鉴定到4个和2个结瘤素基因存在变异位点。本研究将为解析花生根瘤形成发育以及共生固氮提供理论依据。Peanut is an important oil and economic crop of legumes in China.Nodules are important for symbiotic nitrogen fixation in peanut.Dissecting the genetic basis of nodule formation could promote the understanding of nitrogen fixation ability and characteristics of nodules in peanut.However,there are few studies on nodule formation and the genetic basis of nodule formation remained unclear in peanut.In this study,the nodules were investigated in a high generation RIL population and seven lines were identified without nodule.The chlorophyll content,plant height,fresh weight per plant,and dry weight per plant of the lines without nodule were significantly lower than those of parents.Using the previous genetic linkage map based on SSR markers,major QTLs qPNA08 and qPNB07 were identified on chromosome A08 and B07,respectively.The InDel markers in the two major QTLs were developed by the genome resequencing data of parents and performed amplification in the RILs.The interval of QTLs qPNA08 and qPNB07 was reduced to 1.6 Mb and 1.8 Mb,respectively.Meanwhile,the phenotypic variation explained of these two QTLs was increased to 16.4%and 9.9%,respectively.There were four and two nodulin genes variation loci in the two target intervals,respectively.This study provided the understanding for unveiling the genetic basis of formation and development of nodule and symbiotic nitrogen fixation in peanut.

关 键 词：花生 结瘤 QTL定位 连锁分析 

分 类 号：S565.2[农业科学—作物学]