谷子HAK/KUP/KT钾转运蛋白家族全基因组鉴定及其对低钾和高盐胁迫的响应  

Genome-wide identification of the HAK/KUP/KT potassium transporter family in foxtail millet and its response to K+deficiency and high salt stress

作　　者：代书桃[1] 朱灿灿[1] 马小倩 秦娜[1] 宋迎辉[1] 魏昕[1] 王春义[1] 李君霞[1] DAI Shu-Tao;ZHU Can-Can;MA Xiao-Qian;QIN Na;SONG Ying-Hui;WEI Xin;WANG Chun-Yi;LI Jun-Xia(Cereal Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;College of Agriculture,Henan University of Science and Technology,Luoyang 471023,Henan,China)

机构地区：[1]河南省农业科学院粮食作物研究所,河南郑州450002 [2]河南科技大学农学院,河南洛阳471023

出　　处：《作物学报》2023年第8期2105-2121,共17页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-06-14.5-B24);河南省良种联合攻关项目(2022010401);中央引导地方科技发展资金(Z20221341070);河南省农业科学院创新团队和自主创新项目(TD2022033,2022ZC07)资助。

摘　　要：KT/HAK/KUP(HAK)家族是植物中最丰富的钾转运体家族,对植物的生长和环境适应具有重要作用。谷子是抗逆耐瘠研究的模式植物,然而,谷子中HAK家族缺乏系统研究。本研究基于基因组序列信息,鉴定出29个谷子HAK基因(SiHAKs),并对该家族成员的基本特征、蛋白结构、染色体定位、基因复制、表达模式和逆境响应等方面进行了系统分析。结果显示,(1)SiHAKs分为5个进化簇(Cluster I~Cluster V),成员数量分别为11、9、3、3和3。基因结构和蛋白保守基序分析表明,谷子HAK家族具有较高的保守性,不同Cluster的保守性依次为:ClusterIII=Cluster V>Cluster II>Cluster I>Cluster IV。(2)串联复制是SiHAKs扩增的主要原因,15个SiHAKs位于串联重复中。(3)171个转录因子可能结合到不同SiHAKs的启动子上,这些转录因子包含ERF、NAC、MYB和WRKY等家族中的大量成员,可能授予了SiHAKs对非生物胁迫多样的响应机制。(4)基因表达聚类将SiHAKs分成3组:GroupI、Group II和Group III,多数SiHAKs在张谷和豫谷1号2个品种中的表达模式具有一致性;不同Cluster表达水平总体表现为:Cluster III>Cluster V>Cluster II>Cluster I>Cluster IV。(5)根系中表达水平较高的11个SiHAKs用来检测对低钾和高盐胁迫的响应。在低钾胁迫后,8个SiHAKs的表达水平显著升高,1个SiHAK显著降低,2个SiHAKs变化不明显;而高盐胁迫后,3个SiHAKs的表达水平显著升高,2个SiHAKs显著降低,其余6个SiHAKs变化不明显。SiHAK15受到低钾和高盐胁迫的响应最为强烈,其表达量分别为对照的151倍和22倍。(6)基因表达谱的差异反映出不同Cluster间SiHAKs的功能差异。ClusterI主要在根系中表达,可能参与谷子根系K+的吸收;ClusterII不具有组织表达特异性,推测其参与K+的吸收、转运和生长发育等多个生物过程;Cluster III受到低钾和高盐2种胁迫的诱导,显示出维持谷子K+/Na+平衡和抵御盐胁迫的潜在作用;ClusterIV在被检�KT/HAK/KUP(HAK)family is the most abundant potassium(K+)transporter family in plants,which plays important roles in plant growth and environmental adaptation.Foxtail millet(Setaria italic L.Beauv)is a model plant for studies on stress resistance mechanisms.However,the HAK family has not yet been well characterized in foxtail millet.In this study,29 Setaria italic HAK genes(SiHAKs)were identified based on genome-wide sequence information,and the basic characteristics,protein structure,chromosome location,gene replication,expression pattern,and responses to stress were comprehensively analyzed.The results showed as followes:(1)SiHAKs were divided into five phylogenetic clusters(Cluster I–Cluster V),containing 11,9,3,3 and 3 members,respectively.Gene structure and conserved motif analyses indicated that SiHAKs was high conserved,and the conservatisms were as follows:Cluster III=Cluster V>Cluster II>Cluster I>Cluster IV.(2)Tandem replication was the main contribution to the amplification of SiHAKs.15 SiHAKs were located in tandem replication.(3)171 transcription factors,including a large number of members of ERF,NAC,MYB and WRKY families,may bind to the promoter sequences of SiHAKs,which may confer SiHAKs diverse response mechanisms to abiotic stress.(4)SiHAKs were divided into three groups(Group I,Group II,and Group III)by gene expression Cluster Ing.The relative expression patterns of most SiHAKs were consistent in the two varieties(Zhanggu and Yugu 1).The relative expression levels of SiHAKs from the five clusters were generally as follows:Cluster III>Cluster V>Cluster II>Cluster I>Cluster IV.(5)Eleven SiHAKs with high expression levels in root were selected to detect the responses to low K+and high salt stress.Under K+deficiency treatment,eight SiHAKs were markedly upregulated in the expression levels,one SiHAK was significantly decreased,and two SiHAKs had no obvious changes.Under high salt stress,Three Si-HAKs were significantly increased in the expression levels,two SiHAKs were significantly decreased,and th

关 键 词：谷子 钾转运蛋白 KT/HAK/KUP家族 盐胁迫 表达分析 

分 类 号：S515[农业科学—作物学]