机构地区:[1]广东省农业科学院动物科学研究所,畜禽育种国家重点实验室,农业农村部华南动物营养与饲料重点实验室,广东省畜禽育种与营养研究重点实验室,广州510640 [2]湖南生物机电职业技术学院动物科技学院,长沙410128 [3]湖南良平生物科技有限公司,长沙410128
出 处:《动物营养学报》2023年第5期2904-2915,共12页CHINESE JOURNAL OF ANIMAL NUTRITION
基 金:广东省重点领域研发计划项目(2020B0202090004);广东省农业科学院科技人才引进专项资金项目(R2021YJ-YB3012);广州市科技计划项目(202201011186);广东省农业科学院青年科技人员指导协议(R2021QD-024)。
摘 要:本研究旨在评价湿态发酵豆粕在快大型黄羽肉鸡上代谢能和氨基酸回肠消化率。试验1:评估湿态发酵豆粕表观代谢能(AME)和氮校正表观代谢能(AME n)。选取48只30日龄快大型黄羽肉公鸡,随机分2组,每组6个重复,每个重复4只鸡。2组分别饲喂基础饲粮和试验饲粮。预试期3 d,全收粪期4 d。试验2:评估湿态发酵豆粕的氨基酸回肠消化率。选取72只30日龄快大型黄羽肉公鸡,随机分为2组,每组6个重复,每个重复6只鸡。2组分别饲喂无氮饲粮和试验饲粮。试验期3 d。试验1结果表明:饲喂基础下,湿态发酵豆粕的AME和AME n均显著低于普通豆粕(P<0.05);干物质基础下,湿态发酵豆粕的AME和AME n均显著高于普通豆粕(P<0.05)。试验2结果表明,必需氨基酸表观回肠消化率(AID)前4位分别为蛋氨酸(82.55%)、精氨酸(81.66%)、酪氨酸(80.64%)、色氨酸(80.50%),必需氨基酸标准回肠消化率(SID)前4位分别为色氨酸(97.89%)、蛋氨酸(91.26%)、精氨酸(88.20%)、异亮氨酸(87.52%)。非必需氨基酸AID前3位分别为谷氨酸(80.75%)、天冬氨酸(74.17%)、脯氨酸(72.95%),非必需氨基酸SID前3位由高到低分别为脯氨酸(86.56%)、谷氨酸(84.79%)、丝氨酸(83.17%)。由于湿态发酵豆粕水分含量较高,饲喂基础下,氨基酸含量均明显低于普通豆粕。干物质基础下,湿态发酵豆粕氨基酸含量(除色氨酸外)均略低于普通豆粕。必需氨基酸中,湿态发酵豆粕蛋氨酸、苏氨酸和色氨酸SID高于普通豆粕。由此可见,湿态发酵豆粕干物质基础的AME和AME n分别为14.38和13.20 MJ/kg,分别比普通豆粕高25.3%和27.3%,表明豆粕发酵后能提升代谢能。豆粕发酵后,蛋氨酸、苏氨酸和色氨酸SID有所提升。The objective of this study was to evaluate the metabolizable energy and amino acids ileal digestibility of fresh fermented soybean meal in rapidly growing yellow-feathered broilers.Trial 1 was to evaluate the apparent matabolizable energy(AME)and nitrogen corrected apparent matabolizable energy(AME n)of fresh fermented soybean meal.Forty-eight 30-day-old male rapidly growing yellow-feathered broilers were randomly allocated into 2 groups with 6 replicates per group and 4 broilers per replicate.Broilers in 2 groups were fed basal diet and experimental diet,respectively.The pre-experimental period lasted for 3 days,and the total collection period lasted for 4 days.Trial 2 was to evaluate the amino acid ileal digestibility of fresh fermented soybean meal.Seventy-two 30-day-old male rapidly growing yellow-feathered broilers were randomly allocated into 2 groups with 6 replicates per group and 6 broilers per replicate.Broilers in 2 groups were fed nitrogen-free diet and experimental diet,respectively.The experimental period lasted for 3 days.The results of trial 1 showed that on as-fed basis,the AME and AME n of fresh fermented soybean meal were significantly lower than those of regular soybean meal(P<0.05);on dry matter basis,the AME and AME n of fresh fermented soybean meal were significantly higher than those of regular soybean meal(P<0.05).The results of trial 2 showed that the top 4 apparent ileal digestibility(AID)of essential amino acids were methionine(82.55%),arginine(81.66%),tyrosine(80.64%)and tryptophan(80.50%),and the top 4 standardized ileal digestibility(SID)of essential amino acids were tryptophan(97.89%),methionine(91.26%),arginine(88.20%)and isoleucine(87.52%).The AID of non-essential amino acids were glutamate(80.75%),aspartate(74.17%)and proline(72.95%),and the SID of non-essential amino acids were proline(86.56%),glutamate(84.79%)and serine(83.17%).On as-fed basis,the amino acids contents in fresh fermented soybean meal were lower than those in regular soybean meal,due to the high moisture conten
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