饲粮维生素B1添加水平对产蛋期种鹅繁殖性能、血清生殖激素指标、肠道组织形态及盲肠菌群结构的影响  

Effects of Dietary Vitamin B 1 Supplemental Level on Reproductive Performance,Serum Reproductive Hormone Indices,Intestinal Tissue Morphology and Cecal Flora Structure of Breeding Geese during Laying Period

作　　者：李倩 张名爱[1] 王宝维[2] 凡文磊 孔敏[1,2] 汪超[3] LI Qian;ZHANG Ming’ai;WANG Baowei;FAN Wenlei;KONG Min;WANG Chao(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;Institute of High Quality Waterfowl Qingdao Agricultural University,Qingdao 266109,China;Chongqing Academy of Animal Sciences,Chongqing 402460,China)

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]青岛农业大学优质水禽研究所,青岛266109 [3]重庆市畜牧科学院,重庆402460

出　　处：《动物营养学报》2023年第5期3041-3051,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：财政部和农业农村部—国家现代农业产业技术体系资助(CARS-42-14);鹅典型饲料营养价值评定及营养参数研究与应用(cstc2020ngzx0007);2021年度山东省重点研发计划(2021CXGC010805)。

摘　　要：本试验旨在研究饲粮维生素B_(1)添加水平对产蛋期种鹅繁殖性能、血清生殖激素指标、肠道组织形态及盲肠菌群结构的影响。试验选用150只体况相近的34周龄五龙鹅种鹅,随机分为6组,每组5个重复,每个重复5只鹅(1公4母)。各组维生素B_(1)添加水平分别为0(对照组)、1、2、3、4、5 mg/kg。预试期1周,正试期10周。结果表明:1)各组之间种蛋合格率、种蛋受精率、种蛋孵化率差异不显著(P>0.05)。2)各组之间血清催乳素、促黄体生成素、雌二醇、睾酮含量差异不显著(P>0.05)。2 mg/kg维生素B_(1)组血清孕酮含量显著高于对照组和1、3、4、5 mg/kg维生素B_(1)组(P<0.05),2、3 mg/kg维生素B_(1)组血清促卵泡素含量显著高于对照组(P<0.05)。3)2 mg/kg维生素B_(1)组空肠和回肠绒毛高度/隐窝深度显著高于对照组和5 mg/kg维生素B_(1)组(P<0.05)。4)2 mg/kg维生素B_(1)组盲肠菌群的Invsimpson指数显著低于对照组(P<0.05)。在科水平上,2 mg/kg维生素B_(1)组的盲肠弯曲杆菌科(Campylobacteraceac)、纤维单胞菌科(Cellulomonadaceae)、甲基杆菌科(Methylobacteriaceae)相对丰度显著低于对照组(P<0.05)。在属水平上,2 mg/kg维生素B_(1)组的盲肠栖粪杆菌属(Faecalibacterium)相对丰度显著高于对照组(P<0.05)。综上所述,饲粮中添加维生素B_(1)有利于提高产蛋期种鹅血清生殖激素含量,促进肠道组织发育,优化盲肠菌群结构。建议产蛋期种鹅饲粮维生素B_(1)添加水平为2 mg/kg。The purpose of this experiment was to study the effects of dietary vitamin B_(1) supplemental level on reproductive performance,serum reproductive hormone indices,intestinal tissue morphology and cecal flora structure of breeding geese during laying period.A total of 150 thirty-four-weeks-old Wulong breeding geese with similar body conditions were selected and randomly divided into 6 groups with 5 replicates in each group and 5 geese(1 male and 4 female)in each replicate.The vitamin B_(1) supplemental levels in 5 groups were 0(control group),1,2,3,4 and 5 mg/kg,respectively.The pre-experimental period lasted for 1 week,and the experimental period lasted for 10 weeks.The results showed as follows:1)there was no significant difference in the qualified rate of breeding eggs,fertilization rate of breeding eggs and hatchability rate of breeding eggs among all groups(P>0.05).2)There was no significant difference in the contents of prolactin,luteinizing hormone,estradiol and testosterone in serum among all groups(P>0.05).The serum progesterone content of 2 mg/kg vitamin B_(1) group was significantly higher than that of control group and 1,3,4 and 5 mg/kg vitamin B_(1) groups(P<0.05),and the serum follicle-stimulating hormone content of 2 and 3 mg/kg vitamin B_(1) group was significantly higher than that of the control group(P<0.05).3)The jejunum and ileum villus height/crypt depth of 2 mg/kg vitamin B_(1) group was significantly higher than that of control group and 5 mg/kg vitamin B_(1) group(P<0.05).4)The cecal flora Invsimpson index of 2 mg/kg vitamin B_(1) group was significantly lower than that of the control group(P<0.05).At family level,the relative abundances of Campylobacteraceac,Cellulomonadaceae and Methylobacteriaceae in cecum of 2 mg/kg vitamin B_(1) group were significantly lower than those of the control group(P<0.05).At genus level,the relative abundance of Faecalibacterium in cecum of 2 mg/kg vitamin B_(1) group were significantly higher than that of the control group(P<0.05).To sum up,the addition of v

关 键 词：维生素B1 产蛋期 种鹅 血清生殖激素 肠道组织形态 盲肠菌群结构 

分 类 号：S835[农业科学—畜牧学]