N-氨甲酰谷氨酸对荷斯坦公牛胃肠道微生物组成的影响  

Effects of N-Carbamylglutamate on Gastrointestinal Microflora Composition of Holstein Bulls

作　　者：孙雪丽 田雨晴 李树静 张秀江 沈宜钊 李建国[1] 高艳霞[1] 赵超[1] 李秋凤[1] 曹玉凤[1] 肖阳 SUN Xueli;TIAN Yuqing;LI Shujing;ZHANG Xiujiang;SHEN Yizhao;LI Jianguo;GAO Yanxia;ZHAO Chao;LI Qiufeng;CAO Yufeng;XIAO Yang(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,China;Hebei Tianhe Beef Cattle Breeding Co.,Ltd.,Shijiazhuang 050200,China;Animal Husbandry Workstation of Baoding,Baoding 070051,China;Dingxing County Animal Epidemic Prevention and Control Center,Baoding 072650,China)

机构地区：[1]河北农业大学动物科技学院,保定071000 [2]河北天和肉牛养殖有限公司,石家庄050200 [3]保定市畜牧工作站,保定071051 [4]定兴县动物疫病预防控制中心,保定072650

出　　处：《动物营养学报》2023年第5期3081-3092,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家现代农业产业技术体系(CARS-37);河北省二期现代农业产业技术体系肉牛创新团队高效养殖岗位建设项目(HBCT2018130202)。

摘　　要：本试验旨在研究添加不同水平的N-氨基甲酰谷氨酸(NCG)对荷斯坦公牛瘤胃及直肠微生物组成的影响。试验选择45头健康荷斯坦公牛[初始体重(478.00±13.66)kg,12~13月龄],随机分成3组,每组15头。3组公牛分别饲喂在基础饲粮中添加0(Ⅰ组)、15(Ⅱ组)、25 g/(d·头)(Ⅲ组)的NCG的试验饲粮。试验预试期7 d,正试期90 d。结果表明:随着NCG添加水平的增加,瘤胃微生物群多样性未受显著影响(P>0.05),但添加NCG后拟杆菌门的相对丰度呈线性降低趋势(0.050.05);从门水平上分析,添加NCG对直肠微生物区系无显著影响(P>0.05),但随着NCG添加水平的增加,Patescibacteria相对丰度呈二次曲线变化(0.050.05)。综上所述,除瘤胃中瘤胃球菌属1以及直肠中双歧杆菌属、瘤胃球菌科UCG-010之外,添加NCG对瘤胃和直肠微生物相对丰度均无显著影响。This experiment was conducted to investigate the effects of N-carbamylglutamate(NCG)supplementation in different doses on microflora composition in rumen and rectum of Holstein bulls.Forty-five healthy Holstein bulls[initial body weight(478±13.66)kg,12 to 13 months of age]were randomly divided into three groups,and there were 15 bulls in each group,0(groupⅠ),15(groupⅡ)and 25 g/(d•bull)NCG(groupⅢ)were added in a basal diet,respectively.The pre-test period lasted for 7 days and the experimental period lasted for 90 days.Results showed as follows:with the increase of NCG supplemental dose,rumen microbial diversity was not significantly affected(P>0.05),but the relative abundance of Bacteroidetes showed linear decreasing trend(0.05<P<0.10),while the relative abundance of Firmicutes showed linear increasing trend(0.05<P<0.10).At genus level,the relative abundance of Ruminococcus 1 in groupⅡwas significantly higher than that in groupⅠ(P<0.05).The relative abundance of Ruminococcaceae UCG-005 showed increasing trend(P=0.05),and the relative abundance of Succinivibrio showed linear increasing with the increase of NCG supplemental dose(P<0.05),and the relative abundance of Lachnospiraceae 2 showed linear increasing trend with the increase of NCG supplemental dose(0.05<P<0.10).In the rectal microflora,the diversity of rectal microflora showed liner increasing trend with the increase of NCG supplemental dose(P>0.05).At phylum level,the supplement of NCG had no significant effect on rectal microflora(P>0.05),but the relative abundance of Patescibacteria showed quadratic curve change with the increase of NCG dose(0.05<P<0.10).At genus level,the relative abundance of Rikenellaceae RC9 gut group showed liner increasing trend with doses the increase of NCG(0.05<P<0.10),the relative abundance of Bifidobacterium and Ruminococcaceae UCG-010 in groupⅡwas significantly higher than that in groupⅠ(P<0.05),and there was no significant difference compared with groupⅢ(P>0.05).In conclusion,the addition of NCG does not s

关 键 词：N-氨甲酰谷氨酸 荷斯坦公牛 瘤胃微生物 肠道微生物 

分 类 号：S823[农业科学—畜牧学]