黑沙蒿多糖对阿尔巴斯白绒山羊体外瘤胃发酵功能的影响  

Effects of Artemisia ordosica Polysaccharide on in Vitro Rumen Fermentation Function of Albas White Cashmere Goats

作　　者：李书仪 马国强 郭晓宇[1] 赵艳丽[1] 郭咏梅[1] 闫素梅[1] LI Shuyi;MA Guoqiang;GUO Xiaoyu;ZHAO Yanli;GUO Yongmei;YAN Sumei(Key Laboratory of Animal Nutrition and Feed Science at Universities of Inner Mongolia Autonomous Region,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学动物科学学院,动物营养与饲料科学自治区高等学校重点实验室,呼和浩特010018

出　　处：《动物营养学报》2023年第5期3164-3173,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：内蒙古农业大学标志性成果专项资金项目(BZCG201905)。

摘　　要：本试验利用体外批次培养法,通过测定培养各时间点的瘤胃发酵参数并结合多项组合效应指数(MFAEI),研究不同剂量的黑沙蒿多糖对阿尔巴斯白绒山羊体外瘤胃发酵功能的影响,为绒山羊新型替抗产品的开发及黑沙蒿资源的合理利用提供理论支撑。以内蒙古阿尔巴斯白绒山羊为瘤胃液供体动物,采用完全随机区组试验设计,分别向发酵底物中添加0(C组,作为对照组)、0.10%(AOP1组)、0.20%(AOP2组)、0.30%(AOP3组)和0.45%(AOP4组)的黑沙蒿多糖,并分别培养3、6、9、12和24 h后测定瘤胃发酵参数。结果显示:随着黑沙蒿多糖添加剂量的增加,氨态氮(NH3-N)、菌体蛋白(BCP)浓度和产气量均呈显著的一次线性和二次曲线升高(P<0.05),pH和原虫数量均呈显著的一次线性和二次曲线降低(P<0.05)。异丁酸、戊酸和异戊酸浓度随着黑沙蒿多糖添加剂量的增加均呈显著的一次线性和二次曲线升高(P<0.05),丙酸浓度呈显著的一次线性升高(P<0.05),乙酸浓度和乙酸/丙酸值呈显著的一次线性和二次曲线降低(P<0.05);总挥发性脂肪酸(TVFA)浓度各组间差异不显著(P>0.05)。随着黑沙蒿多糖添加剂量的增加,BCP、异丁酸、戊酸和异戊酸浓度的单项组合效应指数(SFAEI)呈显著的一次线性和二次曲线升高(P<0.05),NH3-N和丙酸浓度的SFAEI呈显著的一次线性升高(P<0.05),原虫数量和乙酸浓度的SFAEI呈显著的一次线性和二次曲线降低(P<0.05),MFAEI呈显著的一次线性和二次曲线升高(P<0.05)。MFAEI由高到低排序依次是AOP3组>AOP4组>AOP2组>AOP1组>C组。由此可见,在体外培养条件下,黑沙蒿多糖的添加可改善阿尔巴斯白绒山羊体外瘤胃发酵功能,其中以添加剂量为0.30%时的改善效果为最佳。In this experiment,the effects of different doses of Artemisia ordosica polysaccharide on the in vitro rumen fermentation function of Albas white cashmere goats were investigated by measuring the rumen fermentation parameters at each time point of culture and combining the multiple-factors associative effect index(MFAEI)using the in vitro batch culture method to provide theoretical support for the development of new alternative antibiotic products for cashmere goats and the rational utilization of Artemisia ordosica resources.Using Inner Mongolian Albas white cashmere goat as rumen fluid donor animals in a completely randomized test design,0(C group,as control group),0.1%(AOP1 group),0.2%(AOP2 group),0.3%(AOP3 group)and 0.45%(AOP4 group)of Artemisia ordosica polysaccharide were added to the fermentation substrate and to measure the rumen fermentation parameters after incubated for 3,6,9,12 and 24 h,respectively.The results showed that the ammoniacal nitrogen(NH 3-N),bacterial protein(BCP)concentrations and gas production increased in a significant linear and quadratic curve with the addition dose of Artemisia ordosica polysaccharide increase(P<0.05),and the pH and protozoa number decreased in a significant linear and quadratic curve(P<0.05).Isobutyric acid,valeric acid and isovaleric acid concentrations showed a significant linear and quadratic curve increase with the addition dose of Artemisia ordosica polysaccharide increase(P<0.05),propionic acid concentration showed a significant linear increase(P<0.05),acetic acid concentration and acetic acid/propionic acid value showed a significant linear and quadratic curve decrease(P<0.05),while total volatile fatty acids(TVFA)concentration did not differ significantly among groups(P>0.05).With the addition dose of Artemisia ordosica polysaccharide increase,the single-factor associative effect index(SFAEI)of BCP,isobutyric acid,valeric acid and isovaleric acid concentrations showed a significant linear and quadratic curve increase(P<0.05),the SFAEI of NH 3-N and propion

关 键 词：黑沙蒿多糖 绒山羊 体外瘤胃发酵 产气量 多项组合效应指数 

分 类 号：S816[农业科学—饲料科学]