饲粮中添加丁酸梭菌对伊拉兔盲肠菌群结构及粪便短链脂肪酸含量的影响  

Effects of Dietary Clostridium butyricum on Cecal Flora Structure and Fecal Short Chain Fatty Acids Contents of Ira Rabbits

作　　者：卢佳宁 李可瑶 周丽雯 叶晓星 李亚飞 刘浩宇 王均辉 陈冬金[3] 叶鼎承[3] 方绍明 甘乾福[1] LU Jianing;LI Keyao;ZHOU Liwen;YE Xiaoxing;LI Yafei;LIU Haoyu;WANG Junhui;CHEN Dongjin;YE Dingcheng;FANG Shaoming;GAN Qianfu(College of Animal Science(College of Bee Science),Fujian Agricultural and Forestry University,Fuzhou 350002,China;Fujian Provincial Animal Husbandry Station,Fuzhou 350000,China;Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福州350002 [2]福建省畜牧总站,福州350000 [3]福建省农业科学院畜牧兽医研究所,福州350013

出　　处：《动物营养学报》2023年第5期3249-3265,共17页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：福建省科技厅星火项目(2019S0036);福建农林大学乡村振兴服务团队——草食动物产业服务团队项目(11899170150);福建农林大学科技创新专项基金项目(CXZX2020057A);福建农林大学现代农业装备福建省高校工程研究中心开放基金(MAE-201903)。

摘　　要：本试验旨在研究饲粮中添加丁酸梭菌对伊拉兔盲肠菌群结构及粪便短链脂肪酸(SCFAs)含量的影响。试验选取28日龄体重相近的断奶雄性伊拉兔216只,随机分成4组,每组6个重复,每个重复9只。对照组(CG组)饲喂基础饲粮,试验组分别在基础饲粮中添加200(LC组)、400(MC组)和600 mg/kg(HC组)的丁酸梭菌。丁酸梭菌活菌数为2×108CFU/g。预试期4 d,正试期35 d。结果表明:1)各组盲肠内的优势菌门均为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、疣微菌门(Verrucomicrobia)和放线菌门(Actinobacteria)。2)32和39日龄时,MC组盲肠放线菌门相对丰度显著低于CG组(P<0.05)。60和67日龄时,LC组、MC组和HC组盲肠疣微菌门相对丰度显著低于CG组(P<0.05)。3)53和67日龄时,试验组粪便乙酸、丙酸含量较CG组出现增加趋势。53日龄时,LC组粪便丁酸含量较CG组显著降低(P<0.05);67日龄时,LC组粪便异戊酸含量较CG组显著降低(P<0.05)。4)53日龄时,拟杆菌属(Bacteroides)相对丰度与乙酸含量呈显著正相关(P<0.05);瘤胃球菌科UCG-013(Ruminococcaceae_UCG-013)相对丰度与丙酸含量呈显著负相关(P<0.05),与丁酸含量呈极显著负相关(P<0.01)。67日龄时,瘤胃球菌科NK4A214群(Ruminococcaceae_NK4A214_group)相对丰度与乙酸含量呈显著负相关(P<0.05),Ruminococcaceae_UCG-013相对丰度与丙酸和丁酸含量呈显著负相关(P<0.05)。综上所述,饲粮中添加丁酸梭菌可改变伊拉兔盲肠菌群结构和微生物多样性,改变粪便SCFAs含量,且盲肠菌群相对丰度与粪便SCFAs含量表现出相关性。The purpose of this experiment was to study the effects of dietary Clostridium butyricum on cecal flora structure and fecal short chain fatty acids(SCFAs)contents of Ira rabbits.A total of 216 weaned male Ira rabbits at 28 days of age with similar body weight were randomly divided into 4 groups with 6 replicates in each group and 9 rabbits in each replicate.The control group(CG group)was fed a basal diet,and the experimental groups were fed basal diets supplemented with 200(LC group),400(MC group)and 600 mg/kg(HC group)Clostridium butyricum,respectively.The pre-experimental period lasted for 4 days,and the experimental period lasted for 35 days.The results showed as follows:1)the dominant bacteria in caecum in all groups were Firmicutes,Bacteroides,Verrucomicrobia and Actinobacia.2)At 32 and 39 days of age,the cecal Actinobacia relative abundance of MC group was significantly lower than that of CG group(P<0.05).At 60 and 67 days of age,the cecal Verrucomicrobia relative abundance of LC,MC and HC groups was significantly lower than that of CG group(P<0.05).3)At 53 and 67 days of age,the content of acetic acid and propionic acid in feces of experimental groups were increased compared with CG group.At 53 days of age,the fecal butyric acid content of LC group was significantly lower than that of CG group(P<0.05);at 67 days of age,the fecal isovaleric acid of LC group was significantly lower than that of CG group(P<0.05).4)At 53 days of age,the Bacteroides relative abundance was significant positively correlated with the acetic acid content(P<0.05);the Ruminococcaceae_UCG-013 relative abundance was significant negatively correlated with the propionic acid content(P<0.05),and extremely significant negatively correlated with the butyric acid content(P<0.01).At 67 days of age,the Ruminococcaceae_NK4A214_group relative abundance was significant negatively correlated with the acetic acid content(P<0.05),and the Ruminococcaceae_UCG-013 relative abundance was significant negatively correlated with the propionic acid and buty

关 键 词：丁酸梭菌 伊拉兔 动态变化 微生物区系 短链脂肪酸 

分 类 号：S829.1[农业科学—畜牧学]