不同剂量亚油酸和油酸添加对泌乳奶牛乳腺上皮细胞乳脂合成的影响  

Effects of Different Doses of Linoleic Acid and Oleic Acid Supplementation on Milk Fat Synthesis in Mammary Epithelial Cells of Lactating Dairy Cows

作　　者：白晨[1] 李泽廷 敖长金[1] 哈斯额尔敦[1] BAI Chen;LI Zeting;AO Changjin;KHAS-Erdene(Key Laboratory of Animal Nutrition and Feed Science at Universities of Inner Mongolia Autonomous Region,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古自治区高校动物营养与饲料科学重点实验室,呼和浩特010018

出　　处：《动物营养学报》2023年第5期3280-3289,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：内蒙古自治区自然科学基金项目(2020BS03041)。

摘　　要：本试验旨在研究不同剂量亚油酸和油酸添加对泌乳奶牛乳腺上皮细胞乳脂合成的影响。试验以泌乳奶牛乳腺上皮细胞为研究对象。试验共2部分,试验1以不添加脂肪酸作为对照组,试验组分别添加100μmol/L油酸、100μmol/L亚油酸、100μmol/L油酸+100μmol/L亚油酸。试验2以单独添加100μmol/L油酸作为对照组,试验组在添加100μmol/L油酸的基础上分别添加25、50、75μmol/L亚油酸,每组6个重复。试验测定乳腺上皮细胞活力、甘油三酯相对含量、乳脂合成相关基因的mRNA相对表达量。结果表明:1)试验1,与对照组相比,单独或混合添加油酸和亚油酸均显著提高了乳腺上皮细胞活力(P<0.05),单独添加油酸或亚油酸均显著提高了甘油三酯相对含量(P<0.05),但混合添加未显著影响甘油三酯相对含量(P>0.05);单独添加油酸显著提高了乙酰辅酶A羧化酶(ACACA)、1-单酰甘油-3-磷酸酰基转移酶6(AGPAT6)、3-磷酸甘油酰基转移酶(GPAM)的mRNA相对表达量(P<0.05),单独添加亚油酸显著提高了AGPAT6、固醇调节元件结合蛋白1(SREBF1)的mRNA相对表达量(P<0.05),混合添加等量油酸和亚油酸显著降低了ACACA、SREBF1、分化抗原簇36(CD36)、酰基辅酶A合成酶1(ACSL1)的mRNA相对表达量(P<0.05)。2)试验2,固定油酸添加量,增量添加亚油酸对甘油三酯相对含量无显著影响(P>0.05);100μmol/L油酸和25μmol/L亚油酸混合添加显著增加了ACACA、SREBF1的mRNA相对表达量(P<0.05),显著降低了脂肪酸结合蛋白3(FABP3)的mRNA相对表达量(P<0.05);100μmol/L油酸和50μmol/L亚油酸混合添加显著增加了脂肪酸合成酶(FASN)、硬酯酰辅酶A去饱和酶(SCD)、CD36、AGPAT6、二脂酰甘油酰基转移酶1(DGAT1)、GPAM、磷脂酸磷酸酶(LPIN1)的mRNA相对表达量(P<0.05),显著降低了过氧化物酶体增殖物激活受体γ(PPARG)的mRNA相对表达量(P<0.05);100μmol/L油酸和75μmol/L亚油酸混合添加显著增加了ACACA、FASN、SREThis study aimed to investigate the effects of different doses of linoleic acid and oleic acid supplementation on triglyceride synthesis in mammary epithelial cells of lactating dairy cows.Bovine mammary epithelial cells were used as the research object.The experiment consisted of two parts.In the first experiment,the control group was not supplemented with fatty acids,and the experimental groups were supplemented with 100μmol/L oleic acid,100μmol/L linoleic acid,100μmol/L oleic acid+100μmol/L linoleic acid,respectively.In the second experiment,the control group was supplemented with 100μmol/L oleic acid alone,and the experimental groups were supplemented with 100μmol/L oleic acid and mixed with 25,50 and 75μmol/L linoleic acid.Each group was repeated 6 times.The viability of mammary epithelial cells,the relative content of triglyceride,and the mRNA relative expression levels of genes related to milk fat synthesis were measured.Results showed as follows:1)the results of experiment 1 showed that compared with control group,supplemented with oleic acid and linoleic acid alone or in combination significantly increased the mammary epithelial cell viability(P<0.05);supplemented with oleic acid or linoleic acid significantly increased the relative content of triglyceride(P<0.05),while the mixed supplementation did not affect the relative content of triglyceride(P>0.05);supplemented with oleic acid alone significantly increased the mRNA relative expression levels of acetyl-CoA carboxylase(ACACA),1-monoacylglycerol-3-phosphate acyltransferase 6(AGPAT6),glycerol-3-phosphate acyltransferase(GPAM)(P<0.05).The mRNA relative expression levels of AGPAT6 and sterol regulatory element binding protein 1(SREBF1)were significantly increased by supplementing of linoleic acid alone(P<0.05).Mixed supplemented with equal amounts of oleic and linoleic acid significantly reduced the mRNA relative expression levels of ACACA,SREBF1,differentiation antigen cluster 36(CD36),acyl-CoA synthetase 1(ACSL1)(P<0.05).2)The results of experim

关 键 词：油酸 亚油酸 奶牛乳腺上皮细胞 乳脂合成 

分 类 号：S823[农业科学—畜牧学]