ASE-HPLC-MS/MS检测生物样本中MDMB-4en-PINACA及其代谢物  

Detection of MDMB-4en-PINACA and Metabolites in Biological Samples by ASE-HPLC-MS/MS

作　　者：蔡玉刚 吴永富 周达江[2] 马琦鸿 杜颖川 伍星 王燕军[4] 代勇[3] CAI Yugang;WU Yongfu;ZHOU Dajiang;MA Qihong;DU Yingchuan;WU Xing;WANG Yanjun;DAI Yong(Institute of Criminal Science and Technology,Luzhou Public Security Bureau,Luzhou 646000,Sichuan,China;Institute of Criminal Science and Technology,Chengdu Public Security Bureau,Chengdu 610041,China;Sichuan Police College,Luzhou 646000,Sichuan,China;Criminal Investigation Division of Sichuan Provincial Public Security Department,Chengdu 610041,China)

机构地区：[1]泸州市公安局刑事科学技术研究所,四川泸州646000 [2]成都市公安局刑事科学技术研究所,成都610041 [3]四川警察学院,四川泸州646000 [4]四川省公安厅刑事侦查局,成都610041

出　　处：《刑事技术》2023年第3期241-247,共7页Forensic Science and Technology

基　　金：四川省公安厅科研项目(2021YYCX02);四川省省级大学生创新创业训练项目(S202112212073);四川省科技厅重大科研项目(2020YFS0349)。

摘　　要：为建立快速溶剂萃取–高效液相色谱–三重四极杆串联质谱(ASE-HPLC-MS/MS)检测以肝、肾组织为代表的生物样本中MDMB-4en-PINACA及其代谢物的方法,本文考察了液液萃取、快速溶剂萃取对生物样本中药物的提取效果,用高效液相色谱-三重四极杆串联质谱对生物样本中MDMB-4en-PINACA及代谢物进行分析。结果显示,快速溶剂萃取法回收率最高,在0.5~200 ng/g范围内生物样本中MDMB-4en-PINACA、其水解产物(M1)和脱烷烯基代谢物(M2)浓度与峰面积线性关系良好(r大于0.997 3),MDMB-4enPINACA、M1和M2在肝组织中最低检出限均为0.01 ng/g;MDMB-4en-PINACA、M1和M2在肾组织中最低检出限分别为0.01、0.03和0.02 ng/g。快速溶剂萃取–液相色谱-串联质谱法操作简单、回收率高,可用于生物样本中MDMB-4en-PINACA及其代谢物的检验。To establish an accelerated solvent extraction liquid chromatography-tandem mass spectrometry(ASE-HPLC-MS/MS)method for detecting MDMB-4en-PINACA and its metabolites in biological samples,the effects of liquid-liquid extraction and accelerated solvent extraction on drugs in liver and kidney tissue were investigated.Then the MDMB-4en-PINACA and its metabolites in liver and kidney tissue were analyzed by HPLC-MS/MS.The results showed the ASE had the best performance with a good linear relationship of the MDMB-4en-PINACA,and its hydrolytic metabolite(M1)and dealkenyl metabolite(M2)with the peak area(r>0.9973)in liver and kidney tissue within the range of 0.5–200 ng/g.The LOD values of MDMB-4en-PINACA,M1 and M2 were all 0.01 ng/g in liver tissue.The lower limit of detection values of MDMB-4en-PINACA,M1 and M2 were 0.01,0.03 and 0.02 ng/g in kidney tissue,respectively.The method has the advantages of easy operation and high recovery.It could be concluded that theASE-HPLC-MS/MS can be used to detect the MBMD-4en-PINACAand its metabolites in biological samples.

关 键 词：法医毒物学 MDMB-4en-PINACA 代谢物 快速溶剂萃取 高效液相色谱–三重四极杆串联质谱 

分 类 号：D919.1[医药卫生—法医学]