双酚S对秀丽隐杆线虫精子生成相关基因表达的影响  

Effects of bisphenol S on expression of spermiogenesis genes in C.elegans

作　　者：唐娇 谭剑斌[2] 茅莉娜[3] 张紫虹 李文立[2] 胡帅尔[2] 赵敏[2] TANG Jiao;TAN Jianbin;MAO Lina;ZHANG Zihong;LI Wenli;HU Shuaier;ZHAO Min(Institute of Health Risk Assessment,Guangdong Provincial Institute of Public Health,Guangzhou 511430;Institute of Toxicology,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 511430;Department of Pharmacological Research,Guangdong Provincial Institute of Biological Products and Materia Medica,Guangzhou 510440,Guangdong,China)

机构地区：[1]广东省公共卫生研究院健康风险评估研究室,广东广州511430 [2]广东省疾病预防控制中心卫生毒理所,广东广州511430 [3]广东省生物制品与药物研究所药理研究室,广东广州510440

出　　处：《癌变．畸变．突变》2023年第3期183-186,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省医学科学技术研究基金(A2019562,C2019055);广东省中医药局科研项目(20201042,20202026,20212028)。

摘　　要：目的:探讨双酚S对模式生物秀丽隐杆线虫精子生成相关基因表达的影响。方法:根据双酚S的急性毒性试验结果确定本研究剂量为0、3、30、300μmol/L,对同步化至L4期的线虫进行24 h暴露,测定其子代数目并用实时荧光定量PCR(qPCR)检测akt-1、swm-1、try-5、spe-4、spe-6基因表达水平。结果:秀丽隐杆线虫于双酚S中暴露24 h后,其半数致死浓度(LC50)为2773.672μmol/L;与对照组相比,暴露后的L4期秀丽隐杆线虫在双酚S剂量为3、30、300μmol/L时子代数目均显著降低(P<0.05或P<0.01);akt-1 mRNA在3μmol/L时的表达水平升高(P<0.05);swm-1、try-5、spe-4、spe-6 mRNA在30和300μmol/L时表达水平均升高(P<0.05或P<0.01)。结论:双酚S对秀丽隐杆线虫精子生成相关基因表达有影响。OBJECTIVE:To investigate effects of bisphenol S on expression of spermiogenesis genes in Caenorhabditis elegans(C.elegans).METHODS:Nematodes at L4 stage were exposed to bisphenol S for 24 h(with the concentrations of 0,3,30,300μmol/L based on the acute toxicity test),brood size and expression levels of akt-1,swm-1,try-5,spe-4 and spe-6 genes were detected by real-time fluorescence quantitative PCR.RESULTS:After the 24 h exposure to bisphenol S,the LC50 was determined to be 2773.672μmol/L.Compared to the control group,exposure with the concentrations of 3,30 and 300μmol/L significantly reduced the brood size(P<0.05 or P<0.01).In addition,the mRNA level of akt-1 was increased at the concentration of 3μmol/L(P<0.05);the expression level of swm-1,try-5,spe-4 and spe-6 mRNA were increased in the 30 and 300μmol/L treatment groups(P<0.05 or P<0.01).CONCLUSION:Expressions of spermiogenesis genes in C.elegans were affected from exposure to bisphenol S.

关 键 词：秀丽隐杆线虫 双酚S 精子生成 模式生物 

分 类 号：R994.6[医药卫生—毒理学]