UPLC-Q-TOF-MS/MS代谢组学分析针刺对抑郁症患者血浆代谢物变化的影响  

UPLC-Q-TOF-MS/MS Metabolomics Analysis of the Effect of Acupuncture on Changes in Plasma Metabolites in Patients with Depression

作　　者：曾锐 康冬妮 王省[1] 王红[1,3] 陈斌[1] 汪晶[1] 黄一平[1] ZENG Rui;KANG Dong-ni;WANG Xing;WANG Hong;CHEN Bin;WANG Jing;HUANG Yi-ping(Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China;Department of Pharmacy,Baoji Traditional Chinese Medicine Hospital,Baoji 721000,China;Department of Medical Psychology,Nanjing Brain Hospital,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属中西医结合医院,江苏南京210028 [2]宝鸡市中医医院药学部,陕西宝鸡721000 [3]南京脑科医院医学心理科,江苏南京210029

出　　处：《南京中医药大学学报》2023年第5期411-419,共9页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81803756);国家中医临床研究基地开放课题(JD201710);江苏省中医药研究院自主科研项目(BM2018024-2019013)。

摘　　要：目的运用高效液相色谱-飞行时间质谱联用(UPLC-Q-TOF-MS/MS)技术研究针刺治疗抑郁症的潜在生物标志物,探究针刺抗抑郁的潜在代谢调节机制。方法选取2017年10月至2018年6月南京脑科医院的抑郁症患者25例,随机分为针刺药物组13例和药物组12例(脱落4例),药物组予SSRIs口服治疗,针刺药物组在药物组治疗基础上加予耳内皮刺治疗。收集治疗前后2组患者血浆样品,运用UPLC-Q-TOF/MS检测治疗前后2组患者的血浆代谢产物,比较2组治疗前后代谢物的差异。结果针刺药物组筛选出16个潜在生物标记物,推测针刺药物组通过调节苯丙氨酸代谢、组氨酸代谢、酮体的合成与降解、赖氨酸降解4条代谢途径改善抑郁症患者的异常状态。药物组筛选出9个潜在生物标记物,推测药物组通过调节亚油酸代谢、咖啡因代谢、牛磺酸和亚牛磺酸的代谢、精氨酸和脯氨酸代谢4条代谢途径发挥治疗作用。结论对抑郁症患者代谢物的调控可能是针刺发挥抗抑郁作用的内在机制。OBJECTIVE Using UPLC-Q-TOF-MS/MS to study the potential biomarkers of acupuncture in the treatment of depression,and to explore the potential metabolic regulatory mechanism of acupuncture treatment for depression.METHODS 25 depression patients from Nanjing brain hospital from October 2017 to June 2018 were selected and randomly divided into 13 cases in acupuncture drug group and 12 cases in drug group(with 4 cases fell off).The drug group received oral treatment with SSRIs,while the acupuncture drug group received ear endothelial puncture treatment in addition to the treatment of drug group.Plasma samples were collected from the two groups of patients before and after treatment,and UPLC-Q-TOF-MS/MS was used to detect plasma metabolites before and after treatment.The differences in metabolites between the two groups were compared before and after treatment.RESULTS 16 potential biomarkers were screened out in the acupuncture drug group.It is speculated that the acupuncture drug group can improve the abnormal metabolism of patients with depression by regulating phenylalanine metabolism,histidine metabolism,synthesis and degradation of ketone bodies,and lysine degradation.9 potential biomarkers were screened out in the drug group.It is speculated that the acupuncture drug group improves abnormal metabolism in patients with depression by regulating the four metabolic pathways:phenylalanine metabolism,histidine metabolism,ketone synthesis and degradation,and lysine degradation.CONCLUSION The regulation of metabolites in patients with depression may be the internal mechanism by which acupuncture exerts its antidepressant effect.

关 键 词：针刺 抑郁症 代谢组学 UPLC-Q-TOF-MS/MS 

分 类 号：R246.6[医药卫生—针灸推拿学]