4种常见清热燥湿类中药对肠道菌群及粪便胆汁酸和短链脂肪酸代谢的影响  

Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism

作　　者：李心如 盛先杰 杨琰 安振涛[3] 康安 李慧[3] 葛飞[1] LI Xin-ru;SHENG Xian-jie;YANG Yan;AN Zhen-tao;KANG An;LI Hui;GE Fei(Hai'an Hospital of Traditional Chinese Medicine Affiliated to Hanlin College of Nanjing University of Chinese Medicine,Nantong 226600,China;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Integrated Chinese and Western Medicine Hospital Affiliated to Nanjing University of Chinese Medicine,Jiangsu Academy of Traditional Chinese Medicine,Nanjing 210028,China)

机构地区：[1]南京中医药大学翰林学院附属海安市中医院,江苏南通226600 [2]南京中医药大学药学院,江苏南京210023 [3]南京中医药大学附属中西医结合医院,江苏省中医药研究院,江苏南京210028

出　　处：《南京中医药大学学报》2023年第5期442-451,共10页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省卫健委科研课题面上项目(M2022024);江苏省重点研发计划社会发展项目(BE2022817);南通市卫生健康委员会科研课题(MS2022102)。

摘　　要：目的探究在长期临床等效剂量下4种清热燥湿类中药对正常小鼠肠道菌群、胆汁酸及短链脂肪酸的影响。方法30只Balb/c雄性小鼠被随机分为对照组、苦参组、黄连组、黄柏组和黄芩组,每组各6只。除对照组外,苦参组、黄柏组、黄连组和黄芩组给药剂量分别为2.34、3.12、1.3 g·kg^(-1)和2.6 g·kg^(-1),连续灌胃2周,采用液相色谱-串联质谱法检测粪便中短链脂肪酸和胆汁酸含量,利用16S rRNA高通量基因测序技术分析肠道内容物中菌群结构变化。结果清热燥湿类中药对正常小鼠粪便中6种短链脂肪酸无明显影响。在常见的23种胆汁酸中,与正常组相比,黄芩组、苦参组、黄连组和黄柏组分别有4、3、2、1种胆汁酸显著变化。从肠道菌群丰度变化上看,与对照组相比,除苦参组外,黄连组、黄芩组和黄柏组小鼠厚壁菌门均呈现下降趋势,拟杆菌门均呈现上升趋势,其中黄连组变化更显著。结论苦参、黄柏、黄连和黄芩对正常小鼠长期药效剂量下短链脂肪酸影响较小,但可以通过改变肠道菌群结构影响胆汁酸含量的变化。OBJECTIVE To explore the effects of four kinds of heat-clearing and dampness-drying herbs on intestinal flora,bile acids and short chain fatty acids(SCFAs)in normal mice at long-term clinical equivalent doses.METHODS Thirty Balb/c male mice were randomly divided into control group,Sophora flavescens Ait.(KS)group,Phellodendron chinense Schneid.(HB)group,Coptis chinensis Franch.(HL)group and Scutellaria baicalensis Georgi(HQ)group,with 6 mice in each group.Except for the control group,KS group,HB group,HL group and HQ group were orally administrated at a dosage of 2.34 g·kg^(-1),3.12 g·kg^(-1),1.3 g·kg^(-1) and 2.6 g·kg^(-1),respectively,for two weeks.The contents of short chain fatty acids and bile acids in feces were detected by liquid chromatography tandem mass spectrometry,and the structural changes of microflora in intestinal contents were detected by 16S rRNA high-throughput gene sequencing technology.RESULTS There was no significant effect of heat-clearing and dampness-drying Chinese medicine on the contents of six short-chain fatty acids in the feces of normal mice.Among the 23 common bile acids,when compared with the control group,the numbers of the significantly changed bile acids in HQ group,KS group,HL group and HB group were 4,3,2 and 1,respectively.From the perspective of changes in the abundance of intestinal flora,compared with the control group,except for the KS group,Firmicutes in the HL group,HQ group and HB group all showed a downward trend,and the Bacteroides showed an upward trend,among which the changes in the HL group were the most significant.CONCLUSION Sophora flavescens Ait.,Phellodendron chinense Schneid.,Coptis chinensis Franch.and Scutellaria baicalensis Georgi have little effect on SCFAs in normal mice,but can affect bile acid content by changing the structure of intestinal flora.

关 键 词：清热燥湿 短链脂肪酸 胆汁酸 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]