海南地区冬春作甜瓜病毒病病原鉴定与分析  

Identification and Analysis of Melon Virus Disease Pathogen in Winter and Spring at Hainan Area

作　　者：柳唐镜[1] 邱艳红 陈建军 王锋 许勇[2] 叶云峰[1] 张棵[1] 王敏[3] LIU Tangjing;QIU Yanhong;CHEN Jianjun;WANG Feng;XU Yong;YE Yunfeng;ZHANG Ke;WANG Min(Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;Vegetable Research Institute,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China;Sanya Research Institute,Hainan Academy of Agricultural Sciences,Sanya 572000,Hainan,China)

机构地区：[1]广西壮族自治区农业科学院园艺研究所,广西南宁530007 [2]北京市农林科学院蔬菜研究所,北京100097 [3]海南省农业科学院三亚研究院,海南三亚572000

出　　处：《中国蔬菜》2023年第6期41-47,共7页China Vegetables

基　　金：海南省农业科学院科技创新团队稳定引导经费资助项目(HAAS2022TDYD03);国家西甜瓜产业技术体系项目(CARS-25);海南省院士创新平台科研专项(YSPTZX202116);广西壮族自治区八桂学者项目(2016A11)。

摘　　要：为探明海南地区冬春作甜瓜病毒病的主要病原种类和危害情况,2022—2023年冬春季从海南甜瓜主产区采集34份疑似感染病毒病的甜瓜样品,利用高通量测序(high-throughput sequencing)和聚合酶链式反应(polymerase chain reaction,PCR)技术对甜瓜病毒病的病原种类进行鉴定和分析。结果表明:甜瓜黄斑病毒(melon yellow spot virus,MYSV)、西瓜银斑驳病毒(watermelon silver mottle virus,WSMo V)和中国南瓜曲叶病毒(squash leaf curl China virus,SLCCNV)引起的甜瓜病毒病在海南多个产区普遍发生。其中,MYSV检出率最高,为73.53%;其次为WSMo V,检出率61.76%;且呈现MYSV和WSMo V复合侵染现象,复合侵染率为47.06%。In order to verify the main pathogenic species and hazardous situation of melon virus disease in winter and spring at Hainan region,this paper collected 34 melon samples suspiciously infected by virus disease from main melon producing area during 2022—2023;identified and analyzed the type of melon virus disease pathogen by high-throughput sequencing and polymerase chain reaction(PCR)technology.Results showed that melon yellow spot virus(MYSV),watermelon silver mottle virus(WSMoV)and squash leaf curl China virus(SLCCNV)were prevalent in multiple melon producing areas in Hainan.Among them,the detection rate of MYSV was the highest,being 73.53%,followed by WSMoV with 61.76%detection rate.Moreover,MYSV and WSMoV presented compound infestation phenomenon,with 47.06%mixed infection rate.

关 键 词：甜瓜病毒病 甜瓜黄斑病毒 西瓜银斑驳病毒 中国南瓜曲叶病毒 分类鉴定 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]