不同菜豆种质资源中荚壁纤维含量及组成分析  

Analysis of Pod Wall Fiber Content and Composition in Different Bean Germplasm Resources

作　　者：广宏丽 冯国军[1] 刘大军[1] 杨晓旭 闫志山[1] 刘畅[1] GUANG Hongli;FENG Guojun;LIU Dajun;YANG Xiaoxu;YAN Zhishan;LIU Chang(College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150081,Heilongjiang,China)

机构地区：[1]黑龙江大学现代农业与生态环境学院,黑龙江哈尔滨150081

出　　处：《中国蔬菜》2023年第6期67-76,共10页China Vegetables

基　　金：黑龙江省自然科学基金联合引导项目(LH2019C058)。

摘　　要：菜豆荚壁纤维含量直接影响口感品质,是品种选育的重要指标。为了研究不同种质资源中菜豆荚壁纤维的含量及其组成成分,对158份菜豆商品种质荚壁的粗纤维素、纤维素、半纤维素、木质素含量进行测定分析,其次分别对粗纤维素含量高和低的两个种质不同发育时期的荚壁纤维含量、木质素合成相关酶活性和MDA含量进行测定。结果显示,可将158份菜豆种质按粗纤维素含量的高低分为3类:第Ⅰ类为高纤维含量,第Ⅱ类为中纤维含量,第Ⅲ类为低纤维含量。木质素是引起菜豆荚壁纤维含量升高的主要因子,超氧化物歧化酶(SOD)活性越高越不利于木质素的积累,丙二醛(MDA)含量和过氧化物酶(POD)活性升高则加速其木质化进程。The fiber content of vegetable bean pod wall directly affects the taste quality and is an important indicator for variety selection.In order to study the content and composition of bean pod wall fiber in different germplasm resources,this experiment measured and analyzed the contents of crude cellulose,cellulose,hemicellulose and lignin in pod wall of 158 accessions bean commercial germplasm resources;and then tested the pod wall fiber content,lignin synthesis related enzyme activity and MDA content in 2 germplasm resources with high and low crude cellulose content at different development stages,respectively.The results showed that 158 accessions of bean germplasm resources could be divided into 3 categories according to their crude fiber content:the first category with high fiber content,the second one with medium fiber content and the third one with low fiber content.Lignin is the main factor causing bean pod wall fiber content increase.The higher the activity of superoxide dismutase(SOD),the more unfavorable to the accumulation of lignocellulose.The rising of malondialdehyde(MDA)and peroxidase(POD)enzyme activities can accelerate its lignification process.

关 键 词：菜豆 荚壁纤维组成 纤维素 半纤维素 木质素 

分 类 号：S643.1[农业科学—蔬菜学]