次生代谢相关ceRNA调控网络在胡杨异形叶发生中的作用分析  

The Role of ceRNA Regulatory Networks Related to Secondary Metabolism in the Morphogenesis of Populus euphratica Heterophyll

作　　者：秦少伟 李才林 吴海柱 闫伟伟 何志贵 赵利峰 邢雪琨 Qin Shaowei;Li Cailin;Wu Haizhu;Yan Weiwei;He Zhigui;Zhao Lifeng;Xing Xuekun(School of Leisure and Health,Guilin Tourism University,Guilin,541006;School of Public Health,Guilin Medical University,Guilin,541199)

机构地区：[1]桂林旅游学院休闲与健康学院,桂林541006 [2]桂林医学院公共卫生学院,桂林541199

出　　处：《分子植物育种》2023年第10期3253-3263,共11页Molecular Plant Breeding

基　　金：国家自然科学基金项目(32160235);广西省自然科学基金项目(2021GXNSFAA220090);桂林旅游学院高层次人才启动基金项目(KQ2001);广西自治区本级财政科技计划重点项目(2021AB11081);广西高校中青年教师科研基础能力提升项目(2020KY12008);广西科技基地和人才专项(桂科AD20238094);河南省高等学校青年骨干教师培养计划项目(2019GGJS153)共同资助

摘　　要：为了探究胡杨异形叶发生的分子机制,本研究以胡杨的四种异形叶(线形,披针形,卵圆形,阔卵形)为材料,利用链特异性转录组测序鉴定了胡杨异形叶中的lncRNA和circRNA,建立了它们的表达谱,筛选出差异表达的lncRNA和circRNA,构建了它们的ceRNA调控网络,用Gene Ontology对它们的靶基因(mRNA)进行了注释和分类,确定了参与次生代谢的lncRNA和circRNA,分析了它们在胡杨异形叶发生中的作用。结果表明,在胡杨异形叶中,共有4460个lncRNA(其中442个为新发现)和1149个circRNA(全部为新发现),鉴定出差异表达的371个lncRNA和24个circRNA,根据ceRNA机制和它们的靶基因的功能,发现参与次生代谢的有26个lncRNA和13个circRNA。研究还发现,lncRNA在披针形和卵圆形叶的表达模式最相似,与线形叶的差别最大,而circRNA则在线形和披针形叶的表达模式最相似,与阔卵形叶的差别最大,根据受它们调控并差异表达的mRNA的功能,发现它们主要参与木质素的代谢,还与萜类和酚类的代谢以及发育过程相关。本研究表明lncRNA和circRNA介导的ceRNA网络主要通过调控木质素代谢参与胡杨异形叶的发生,同时激素和叶绿素代谢调控对阔卵形叶的产生至关重要,为揭示植物叶形发育的机制提供一个新的角度和策略。To investigate the molecular mechanism of Populus euphratica heterophyll morphogenesis,in this study,four heteromorphic leaves of P.euphratica linear,lanceolate,oval,and broad oval were used as materials,their lncRNAs and circRNAs were identified by chain specific transcriptome sequencing,and the expression profiles of RNAs were also established.Moreover,the differentially expressed lncRNAs and circRNAs were screened out,their ceRNA regulatory networks were constructed,and their functions were also annotated according to their target genes by Gene Ontology.At last,the lncRNAs and circRNAs involved in secondary metabolism were further identified,and their roles in the development of leaf shapes were analyzed.The results showed that there were 4460 lncRNAs(442 novel lncRNAs)and 1149 novel circRNAs in heterologous leaves of P.euphratica,among them,371 lncRNAs and 24 circRNAs were differentially expressed.According to ceRNA mechanism and the function of their target genes,there were 26 lncRNAs and 13 circRNAs involved in secondary metabolism.In addition,lncRNAs had the most similar expression patterns in lanceolate and oval leaves,and were significant difference from linear leaves;while circRNAs had the most similar expression patterns in linear and lanceolate leaves,and were significant difference from broad-ovate leaves.According to the function of differentially expressed mRNAs which regulated by them,it was found that these lncRNAs and circRNAs were mainly involved in lignin metabolism,and them were also related to the metabolism and development of terpenoids and phenols.The present study demon strated that lncRNA-and circRNA-mediated ceRNA networks are mainly involved in the formation of P.euphratica heteromorphic leaves by regulating lignin metabolism,and that the regulation of hormone and chlorophyll metabolism is crucial for the morphogenesis of broad oval leaves.These results providing a new perspective and strategy for revealing the mechanism of leaf shape development in plants.

关 键 词：胡杨异形叶 次生代谢 ceRNA lncRNA circRNA 调控 

分 类 号：S792.11[农业科学—林木遗传育种]