复合菌群构建及其对马铃薯渣动物饲料的发酵条件优化  

Construction of complex flora and optimization of fermentation conditions for potato residue animal feed

作　　者：连洁 王薇[1] 梁瑞雪 侯艳 戚月娜 刘晓飞[1] LIAN Jie;WANG Wei;LIANG Rui-xue;HOU Yan;QI Yue-na;LIU Xiao-fei

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028

出　　处：《饲料研究》2023年第8期68-73,共6页Feed Research

基　　金：哈尔滨商业大学青年学术骨干项目(项目编号:2020CX03)。

摘　　要：试验旨在提高马铃薯渣的饲用价值。试验将实验室保藏酶活较高的两株菌株AAG-17和CPA-3-4进行复合菌群的构建。采用单因素结合响应曲面法对发酵条件进行优化,并对降解前后的马铃薯渣进行红外和电镜观察。结果显示,培养基初始pH值为7、发酵转速为160 r/min、接菌量为2%、接菌比例(CPA-3-4∶AAG-17)为3∶1,马铃薯渣降解率理论值为36.58%,重复试验得到降解率为36.90%。在此条件下对马铃薯渣进行降解,红外和扫描电镜结果均表明复合菌群对马铃薯渣具有降解作用。研究表明,试验确定了复合菌群发酵马铃薯渣动物饲料的最佳条件,发酵后的马铃薯渣动物饲料中营养价值得到提升。The experiment was to improve the feeding value of potato residue.Two strains AAG-17 and CPA-3-4 with high enzyme activity preserved in laboratory were used to construct composite flora,the fermentation conditions were optimized by single factor combined with response surface method,and the potato residue before and after degradation was observed by infrared and electron microscopy.The results showed that when the initial pH value of 7,the fermentation revolution of 160 r/min and the inoculated-pathogen quantities of 2%,the inoculation ratio(CPA-3-4:AAG-17)was 3:1,the theoretical value of degradation rate of potato residue was 36.58%,and the repeated test results showed that the degradation rate was 36.90%.The results of SEM and Fourier IR on potato residue before and after degradation showed that complex flora had significant degradation effect on potato residue.The study indicates that the experiment determines the optimum condition of compound flora fermentation of potato residue animal feed,and the nutritional value of ferment potato residue animal feed is improved.

关 键 词：复合菌群 马铃薯渣 响应曲面优化 降解率 

分 类 号：S816.6[农业科学—饲料科学]