一种超声引导下快速建立小鼠急性心肌梗死模型的方法  

A rapid method for acute myocardial infarction model with assistance of echocardiography in mice

作　　者：张运行 杨虹波 王艳艳 钟鸣 王婷 Zhang Yunhang;Yang Hongbo;Wang Yanyan;Zhong Ming;Wang Ting(Department of Critical Care Medicine,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Department of Cardiology,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院重症医学科,上海200032 [2]复旦大学附属中山医院心内科,上海200032

出　　处：《中华实验外科杂志》2023年第3期580-583,共4页Chinese Journal of Experimental Surgery

基　　金：上海市"医苑新星"青年医师培养计划(2021-99)。

摘　　要：目的探讨超声心动图辅助下构建小鼠急性心肌梗死(AMI)模型的方法。方法SPF级C57BL/6小鼠80只,超声心动图引导下将针电极置入到左冠状动脉前降支(LAD),10瓦特电凝5~10 s阻断血流诱导AMI;假手术组(Sham)步骤同上,不进行电凝。记录建模前后心电图;术后1天检测血浆游离肌钙蛋白T(cTnT),升主动脉注入Microfil显影冠脉;28 d后超声测量左室射血分数(LVEF)、收缩末期容积(LVESV)、舒张末期容积(LVEDV)、整体纵向应变(LVGLS)、面积变化分数(LVFAC);心脏切片马松染色。在LAD远、中、近段分别阻断血流构建小、中等及大面积AMI。两组间比较采用t检验,两组以上比较采用one-way ANOVA。结果建模后心电图ST段抬高,术后第1天cTnT显著升高,梗死区域清晰且无冠脉血管显影。术后28 d心超发现,AMI小鼠LVGLS明显高于Sham小鼠(-5.6±1H7比-17.1±1.8,t=12.17,P<0.01);术后28 d马松染色见纤维瘢痕形成。构建小、中等及大面积AMI模型,cTnT随AMI面积扩大而逐渐升高[Sham:(280.0±145.3)pg/ml;小:(5811.0±987.3)pg/ml;中等:(6975.2±1338.4)pg/ml;大:(9590.5±2173.8)pg/ml,F=49.69,P<0.01]。AMI后28 d随心梗面积增大,心脏重量逐渐增加(Sham:6.12±0.25;小:6.31±0.44;中等:6.93±0.67;大:7.69±1.05,F=7.31,P<0.01)。结论应用超声心动图辅助可快速制备小鼠AMI模型,该方法创伤小,重复性好。Objective To develop a rapid method for establishing mouse acute myocardial infarction(AMI)under echocardiography.Methods SPF grade C57BL/6 mice were fixed on an ultrasound operating table.A needle electrode tip was placed into the left coronary artery descending branch(LAD)vessel under echocardiography.An electrocoagulation intervention of 10 watts was applied for 5 to 10 s.Electrocardiogram was recorded.The plasma free troponin T(cTnT)concentration was detected.To visualize LAD,Microfil was injected into LAD.On day 28,left ventricular ejection fraction(LVEF),end-systolic volume(LVESV),end-diastolic volume(LVEDV),and global longitudinal strain(LVGLS)were analyzed.Heart was sliced and stained with Masson.Results ECG showed elevation of ST segment.Boundaries of infarct area were clear.No vessel could be visualized.Heart function in the infarct mice was significantly deteriorated,compared to that in the Sham with GLS(-5.6±1.7 vs.-17.1±1.8,t=12.17,P<0.01).On day 28,a thin fibrous scar could be observed.In the induction of different MI sizes,We observed different cTnT levels on day 1[Sham:(280.0±145.3)pg/ml;Small:(5811.0±987.3)pg/ml;Middle:(6975.2±1338.4)pg/ml;Large:(9590.5±2173.8)pg/ml,F=49.69,P<0.01]and different heart weight on day 28(Sham:6.12±0.25;Small:6.31±0.44;Middle:6.93±0.67;Large:7.69±1.05,F=7.31,P<0.01).Conclusion This protocol can be quickly accomplished under echocardiography,and it is less invasive and repetitive.

关 键 词：急性心肌梗死 超声心动图 电凝 小鼠 

分 类 号：R542.22[医药卫生—心血管疾病] R-332[医药卫生—内科学]