妊娠纹修复经皮共输送纳米载体细胞功效研究  

Cellular efficacy study of percutaneous co-delivery nanocarriers for stretch mark repair

作　　者：邓文娟 罗丹 陈家铃[2,3] 孙永 郭莉莉 刘雅文 洪延涵 刘卫 DENG Wen-juan;LUO Dan;CHEN Jia-ling;SUN Yong;GUO Li-li;LIU Ya-wen;HONG Yan-han;LIU Wei(Guangzhou Natural Country Biotechnology Co.,Ltd.,Guangzhou 511300,China;Wuhan Bestcarrier Biotechnology Co.,Ltd.,Wuhan 430075,China;National Engineering Research Center for Nanomedicine,Huazhong University of Science and Technology,Wuhan 430075,China;College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China)

机构地区：[1]广州天然国度生物科技有限公司,广东广州511300 [2]武汉百思凯瑞生物科技有限公司,湖北武汉430075 [3]华中科技大学国家纳米药物工程技术研究中心,湖北武汉430075 [4]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《精细与专用化学品》2023年第5期16-21,共6页Fine and Specialty Chemicals

摘　　要：采用高压均质技术制备了载有二棕榈酰羟脯氨酸/腺苷/羟基积雪草苷/棕榈酰五肽-4等4种妊娠纹修复活性成分的经皮共输送纳米载体(妊娠纹修复纳米载体),通过体外细胞实验探究其妊娠纹修复功效。采用CCK-8法检测人皮肤成纤维细胞(HSF)存活率,活性氧试剂盒检测HSF细胞胞内ROS水平,ELISA试剂盒测定COLⅠ、COLⅢ、ELN、HA、MMP-1以及MMP-3水平,评价妊娠纹修复纳米载体在皮肤妊娠纹修复方面的作用。细胞功效实验结果显示,与游离活性物比较,妊娠纹修复纳米载体可显著促进HSF细胞增殖,对H_(2)O_(2)诱导氧化损伤的HSF细胞存活率显著上升,显著降低HSF细胞胞内ROS水平,且能促进HSF细胞分泌COLⅠ、COLⅢ、ELN及HA,显著抑制MMP-1和MMP-3的活性。Transdermal co-delivery nanocarriers containing four active ingredients of dipalmitoyl hydroxyproline/adenosine/hydroxy cumene/palmitoyl pentapeptide-4for stretch mark repair were prepared by high pressure homogenization technique(stretch mark repair nanocarrier),exploring the efficacy of stretch mark repair through in vitro cellular experiments.Detection of human skin fibroblast(HSF)survival rate by CCK-8method and intracellular ROS level in HSF cells by reactive oxygen species kit.Determination of COLⅠ,COLⅢ,ELN,HA,MMP-1and MMP-3levels by ELISA kits to evaluate the role of stretch mark repair nanocarriers in skin stretch mark repair.The results of cell efficacy experiments showed that,compared with free actives,stretch mark repair nanocarriers significantly promoted HSF cell proliferation,significantly increased the survival rate of HSF cells with H_(2)O_(2)-induced oxidative damage,significantly reduced the intracellular ROS level of HSF cells,and promoted the secretion of COLⅠ,COLⅢ,ELN and HA by HSF cells,and significantly inhibited the activity of MMP-1and MMP-3.

关 键 词：二棕榈酰羟脯氨酸 腺苷 羟基积雪草苷 棕榈酰五肽-4 纳米载体 妊娠纹修复 

分 类 号：TQ469[化学工程—制药化工]