液相芯片技术在检测致病相关基因中应用进展  

Application progress of liquid chip technology in the detection of disease-related genes

作　　者：杨娟 彭启凤 刘平[2] 张人俊[3] 卢美 李吉达 王幼明[2] 李岩 尹燕博[4,5] 张毅 YANG Juan;PENG Qi-feng;LIU Ping;ZHANG Ren-jun;LU Mei;LI Ji-da;WANG You-ming;LI Yan;YIN Yan-bo;ZHANG Yi(College of Public Health,Zunyi Medical University,Zunyi 563000,China;China Animal Health and Epidemiology Center,Qingdao 266032,China;Guiyang Animal Disease Prevention and Control Center,Guiyang 550008,China;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;Qingdao Bolong Experimental Animal Company,Qingdao 266225,China)

机构地区：[1]遵义医科大学公共卫生学院人兽共患研究所,贵州遵义563000 [2]中国动物卫生与流行病学中心,山东青岛266032 [3]贵州省动物疫病预防控制中心,贵州贵阳550008 [4]青岛农业大学动物医学院,山东青岛266109 [5]青岛博隆实验动物有限公司,山东青岛266225

出　　处：《生物技术》2023年第2期254-259,267,共7页Biotechnology

基　　金：贵州省科学技术基金项目(黔科合支撑[2021]一般163,黔科合基础-ZK[2023]重点057,黔科合支撑[2020]1Y037号);中动卫科技创新基金项目(DW2021010);青岛市关键技术公共及产业化示范类项目(23-1-4-xxgg-21-nsh)。

摘　　要：液相芯片是一种重要的高通量分子检测技术,能够快速对一个样本进行多重检测和分析。该文综述了近年来液相芯片检测人类和模式动物的致病基因的应用,发现该技术能够对遗传性疾病、心血管疾病等致病相关基因及其在组织中的表达进行了定量检测,为临床诊疗和致病机制的研究提供了有力的支撑;还能够筛查实验动物的相关致病基因,以期为深入研究疾病与致病基因之间的关系,以及疾病动物模型的构建提供参考。Liquid chip is an important high-throughput molecular detection technology,which can multiply detect and analyze different samples.This paper mainly introduce the application of liquid chip in the research of detecting pathogenic genes in humans and model animals,and it was found that this technology can quantitatively detect the pathogenic genes and their expression in tissues,such as genetic diseases and cardiovascular diseases,which could provide support for clinical diagnosis and pathogenesis research;moreover,it can also screen the pathogenic genes of experimental animals.Consequently,it cloud provide references for further study in the relationship between diseases and pathogenic genes,and the construction of disease animal models.

关 键 词：液相芯片 基因 疾病 动物模型 

分 类 号：R318[医药卫生—生物医学工程]