高效液相色谱法同时测定五味子中的4种木脂素成分  

The Simultaneous Determination of Four Lignans in Schisandra Chinensis by HPLC

作　　者：唐琪 欧阳云 和薛 王辉[2] 郑济芳 徐小娜 TANG Qi;OUYANG yun;HE Xue;WANG Hui;ZHENG Jifang;XU Xiaona(SGuilin Medical College,Guilin 541199,China;South China University,Hengyang 421001,China;The Guangxi Key Laboratory of Environmental Exposomics and Entire Lifecycle Health,Guilin 541199,China)

机构地区：[1]桂林医学院【公共卫生学院】,广西桂林541199 [2]南华大学【公共卫生学院】,湖南商衡阳421001 [3]广西环境暴露组学与全生命周期健康重点实验室,广西桂林541199

出　　处：《品牌与标准化》2023年第3期103-105,共3页

基　　金：广西自然科学基金项目(2020JJA140132);广西高校中青年教师科研基础能力提升项目(2023KY0527);桂林医学院人才引进科研项目(20501020028)。

摘　　要：目的:建立高效液相色谱法(HPLC)同时测定五味子中五味子醇甲等4种木脂素成分的含量。方法:采用Sepax C18(250 mm×4.6 mm,5μm)色谱柱,甲醇-乙腈-水流动相梯度洗脱,流速1.0 mL/min,柱温30℃,进样量20μL,检测波长220 nm。结果:五味子醇甲、五味子酯甲、五味子甲素和五味子乙素分别在0.365~13.194 mg/g、0.388~14.853 mg/g、0.442~16.630 mg/g、0.313~11.882 mg/g浓度范围内呈良好的线性关系,回收率、精密度、稳定性等试验结果均符合方法学测定要求。12批不同产地五味子的4种木脂素成分含量有差异,RSD为0.76%~3.28%。结论:建立的高效液相色谱法可用于同时分析五味子中的4种木脂素成分。Objective:To establish a high performance liquid chromatography(HPLC)method for simultaneous determination Schizandrol A and other three lignans in Schisandra Chinensis.Methods:HPLC separations were carried out on a Sepax C18 column(250 mm×4.6 mm,5μm)by gradient elution using methanol,acetonitrile and water as mobile phase.The flow rate was 1.0 mL/min,and the column temperature was 30°C.The injection volume was 20μL and the detection wavelength was 220 nm.Results:The linear relationships of Schizandrol A,Schisantherin A,Schizandrin A and Schizandrin B were good within the ranges of 0.36~13.20,0.39~14.86,0.44~16.63,and 0.32~11.88 mg/g,relatively.The results of recovery,precision,stability and so on all met the requirements of methodological determination.The content of the four lignans tested in 12 batches of Schisandra Chinensis samples from different producing areas was different,with the RSD of 0.76%~3.28%.Conclusin:The established method can be applied to determine the four lignans in Schisandra Chinensis simultaneously.

关 键 词：五味子 高效液相色谱法 五味子醇甲 五味子酯甲 五味子甲素 五味子乙素 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]