不同强度运动预防高脂膳食大鼠心肌脂质沉积效果及对miR-145-5p、KLF5、PPARα表达的影响  

Roles of exercise on preventing myocardial lipid deposition and miR-145-5p,KLF5,PPARαin high-fat diet rats

作　　者：吴卫东[1,2,3] 王伟欢 刘帅 何玉秀 WU Weidong;WANG Weihuan;LIU Shuai(Hebei Normal University,Sports Academy,Shijiazhuang,Hebei,050024)

机构地区：[1]河北师范大学体育学院,石家庄市050024 [2]河北省人体运动生物信息测评重点实验室 [3]郑州大学体育学院

出　　处：《中国康复医学杂志》2023年第5期589-599,共11页Chinese Journal of Rehabilitation Medicine

基　　金：河北师范大学科技类研究基金项目(L2020Z08);河北省博士研究生创新资助项目(CXZZBS2020071);河南省科技攻关项目(202102310324)。

摘　　要：目的:观察12周中等强度持续运动(moderate-intensity continuous training,MICT)与高强度间歇运动(high intensity interval training,HIIT)预防高脂膳食大鼠心肌脂质沉积效果的异同,以及对miR-145-5p、KLF5、PPARα表达的影响,从而探讨其可能机制。方法:5周龄雄性SD大鼠随机分为普通膳食安静组(C组)、高脂膳食安静组(F组)、高脂膳食MICT组(M组)、高脂膳食HIIT组(H组),每组8只。12周跑台训练干预结束后,超声心动图检测大鼠心脏结构与功能;光镜和透射电镜观察大鼠心肌脂质沉积;检测大鼠血清、心肌脂质含量;RT-PCR、Western Blot和免疫荧光双标记检测大鼠心肌miR-145-5p、KLF5、PPARα的表达情况。结果:(1)与C组比较,F组大鼠心肌结构、功能受损;心肌纤维松弛,脂质沉积增多;血清及心肌脂质含量增加(P<0.05);心肌组织miR-145-5P表达升高(P<0.05),KLF5、PPARαm RNA与蛋白表达水平降低(P<0.05);KLF5、PPARα阳性细胞数降低(P<0.01)。(2)与F组比较,M、H组大鼠心肌结构、功能均改善;心肌纤维紧密,脂质沉积减少;血清及心肌脂质含量减少(P<0.01);心肌组织miR-145-5P表达降低(P<0.05),KLF5、PPARαm RNA与蛋白表达水平增加(P<0.05);KLF5、PPARα阳性细胞数增加(P<0.01)。(3)与M组比较,H组大鼠心肌脂质沉积减少;血清HDL含量增加(P<0.01),心肌TG含量降低(P<0.05);KLF5、PPARα阳性细胞数增加(P<0.01)。结论:12周MICT与HIIT均能预防高脂膳食大鼠心肌脂质沉积,大鼠心肌miR-145-5p、KLF5、PPARα表达水平的变化可能是运动影响心肌脂质代谢的机制之一,HIIT预防效果较好,可能与该信号通路的活化程度有关。Objective:To observe the effect of moderate-intensity continuous training(MICT)and high intensity interval training(HIIT)on preventing myocardial lipid deposition in high-fat diet rats at 12 weeks,and to explore the role of miR-145-5p,KLF5,PPARαexpression.Method:Five-week-old male SD rats were randomly assigned to one of four groups:normal diet quiet(Group C),high fat diet quiet(Group F),high fat diet MICT(Group M),and high fat diet HIIT(Group H),each with eight rats.After a 12-week treadmill exercise intervention,echocardiography was used to detect the rat cardiac structure and function;light microscopy and transmission electron microscopy were used to observe myocardial lipid deposition;and serum and myocardial lipid levels were measured.The expressions of miR-145-5p,KLF5 and PPAR in rat myocardia were detected by RT-PCR,Western Blot and immunofluorescence.Result:Compared with group C,myocardial structure and function were impaired in group F;myocardial fibers were relaxed and lipid deposition was increased;serum and myocardial lipid content increased(P<0.05).The expression of miR-145-5P was increased(P<0.05),and the mRNA and protein expressions of KLF5 and PPARαwere decreased(P<0.05).The number of KLF5 and PPARαpositive cells decreased(P<0.01).Compared with group F,myocardial structure and function were improved in groups M and H;the myocardial fibers were compact and lipid deposition was reduced.Serum and myocardial lipid content decreased(P<0.01).The expression of miR-145-5P was decreased(P<0.05),and the mRNA and protein expressions of KLF5 and PPARαwere increased(P<0.05).The number of cells expressing KLF5 and PPARαincreased(P<0.01).Compared with group M,myocardial lipid deposition was decreased in group H;Serum HDL content increased(P<0.01),while myocardial TG content decreased(P<0.05).The number of cells expressing KLF5 and PPARαincreased(P<0.01).Conclusion:Both MICT and HIIT at 12 weeks can prevent myocardial lipid deposition in rats on the high fat diet,changes in the expression levels of miR-145-

关 键 词：中等强度持续运动 高强度间歇运动 高脂膳食 心肌脂质沉积 miR-145-5p KLF5 

分 类 号：R493[医药卫生—康复医学] R541[医药卫生—临床医学]