基于SCoT分子标记的苇状羊茅品种遗传关系及指纹图谱分析  

Analysis of Genetic Relationships and Fingerprints of Festuca arundinacea Based on SCoT Molecular Markers

作　　者：赵旭 陈仕勇[2] 余静菠 张慧丽 关皓 周青平 ZHAO Xu;CHEN Shiyong;YU Jinbo;ZHANG Huili;GUAN Hao;ZHOU Qingping(Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China;College of Animal&Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学青藏高原研究院,四川成都610041 [2]西南民族大学畜牧兽医学院,四川成都610041

出　　处：《中国草地学报》2023年第5期1-8,共8页Chinese Journal of Grassland

基　　金：四川省“十四五”饲草育种攻关项目(2021YFYZ0013);国家牧草产业技术体系(CARS34);西南民族大学研究生创新型科研项目(ZD2022144)资助。

摘　　要：采用目标起始密码子多态性标记(SCoT)对13个苇状羊茅品种进行遗传多样性、遗传结构及指纹图谱分析,以期为苇状羊茅种质资源鉴定、野生种质资源驯化及新品种培育提供理论依据。从80个SCoT引物中筛选出多态性较好、条带清晰且重复性高的引物15个进行扩增,13个苇状羊茅品种共扩增出181条条带,平均每个引物扩增12.07条。其中,多态性条带共176条,不同引物扩增的多态性条带数在7~16条之间,平均每个引物扩增多态性条带为11.73条,多态性条带比率(PPB)为97.24%;有效等位基因数范围在1.311~1.642之间,平均为1.511;基因多样性指数范围在0.184~0.357之间,平均为0.294;Shannon指数范围在0.276~0.518之间,平均为0.435。供试的13个品种间的遗传距离在0.0444~0.1401之间,平均值为0.1003;基于遗传距离的UPGMA聚类分析可将13个品种分为两类,与遗传结构分析结果一致。分子方差分析结果表明,总的遗传变异中有14%发生在品种间,有86%发生在品种内。引物SCoT10、SCoT56、SCoT80可以完全区分供试苇状羊茅品种,采用SCoT10引物扩增出的7个多态性位点成功构建了13个苇状羊茅品种的指纹图谱。In this study,the genetic diversity,genetic structure and fingerprints of 13 Festuca arundinacea cultivars were analyzed using start codon target polymorphism(SCoT)marker,in order to provide theoretical basis for the identification of germplasm resources,domestication of wild germplasm resources,and cultivation of new varieties of Festuca arundinacea.Fifteen primers with good polymorphism,clear bands and high repeatability were selected out from 80 SCoT primers for amplification.A total of 181 bands were amplified from 13 Festuca arundinacea cultivars,with an average of 12.07 bands amplified per primer.Among them,there were a total of 176 polymorphic bands,with 7 to 16 polymorphic bands amplified by different primers.The average number of polymorphic bands amplified by each primer was 11.73,and the percentage of polymorphic bands(PPB)was 97.24%.The effective number of alleles(Ne)ranged from 1.311 to 1.642,with an average of 1.511.The values of Nei’s gene diversity(H)ranged from 0.184 to 0.357,with an average of 0.294.The Shannon’s information index(I)ranged from 0.276 to 0.518,with an average of 0.435.The genetic distance among the 13 tested cultivars ranged from 0.0444 to 0.1401,with an average of 0.1003.The UPGMA cluster analysis based on genetic distance can divided 13 cultivars into two groups,which was consistent with the results of genetic structure analysis.AMOVA results showed that 14%of the total genetic variation was inter-varietal and 86%was intravarietal.Primers SCoT10,SCoT56 and SCoT80 could completely distinguish the tested Festuca arundinacea varieties.The fingerprints of 13 varieties of Festuca arundinacea were successfully constructed using 7 polymorphic loci amplified by primers SCoT10.

关 键 词：苇状羊茅 SCoT 指纹图谱 亲缘关系 

分 类 号：S543.9[农业科学—作物学]